Human beta-Arrestin 2 Antibody

Catalog #: AF4564 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4564
AF4564-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human beta-Arrestin 2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human beta -Arrestin 2 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human beta -Arrrestin 1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human beta -Arrestin 2
Met1-Cys409
Accession # P32121

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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beta ‑Arrestin 2 in Human Brain. beta ‑Arrestin 2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 10 µg/mL Human beta ‑Arrestin 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4564) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-Arrestin 2

The Arrestin family consists of four members: Arrestin 1 (visual Arrestin), Arrestin 2 ( beta -Arrestin 1), Arrestin 3 ( beta -Arrestin 2), and Arrestin 4 (cone Arrestin). While visual and cone Arrestins are found almost exclusively in the retina, beta -Arrestins 1 and 2 are ubiquitously expressed, and were initially described as negative regulators of G protein-coupled receptor (GPCR) signaling. More recently, beta -Arrestins have been determined to serve as scaffolds for various signaling pathways, including the MAPK cascades activating ERK2, p38 alpha, and JNK3. These beta -Arrestin scaffolds tie together the appropriate kinases in series, forming a discreet signaling module that localizes components to specific subcellular environments and facilitates greater kinase activation.

Entrez Gene IDs

409 (Human); 216869 (Mouse); 25388 (Rat)

Alternate Names

ARB2; ARR2; ARRB2; arrestin 3; Arrestin beta-2; arrestin, beta 2; BARR2; betaArrestin 2; beta-Arrestin 2; beta-arrestin-2; DKFZp686L0365