Human Biglycan Antibody

Catalog #: MAB2667
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2667
MAB2667-SP

All Biglycan Antibodies

Product Details

Citations (2)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Biglycan Antibody Summary

Species Reactivity

Human

Specificity

Detects human Biglycan in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 303203

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Biglycan
Asp38-Lys368
Accession # P21810

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Biglycan (Catalog # 2667-CM)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Biglycan

Biglycan, also known as PG I, is a secreted chondroitin/dermatan sulfate proteoglycan in the small leucine-rich proteoglycan (SLRP) family. SLRP family members are characterized by N-terminal and C-terminal cysteine-rich regions that flank a central region containing 10 - 12 tandem leucine-rich repeats (LRRs). Biglycan function is important in the development and maintenance of many tissues (1, 2). The human Biglycan cDNA encodes a 368 amino acid (aa) precursor with a 19 aa signal sequence and a 18 aa propeptide that is cleaved by BMP-1 (3). Mature Biglycan contains N-linked glycosylation in addition to two glycosaminoglycan (GAG) chains (4). The 45 kDa core protein is approximately one third the molecular weight of the fully glycanated form and can assemble into noncovalently-associated dimers (5). Human Biglycan shares 97% aa sequence identity with bovine, mouse, and rat Biglycan and 57% aa sequence identity with human Decorin. Biglycan binds several matrix proteins, including fibrillar collagens, Matrilin-1, and beta IG-H3 (6 - 8). Its multiple LRRs, N-linked glycosylation, and GAG chains mediate distinct interactions that enable extensive crosslinking and stabilization of the collagen matrix (6 - 8). Mature Biglycan can be further cleaved by proteases, resulting in non-glycanated fragments (9). In osteoarthritis, MMP-13-induced breakdown of Biglycan likely interferes with collagen crosslinking and contributes to cartilage degradation (10). Biglycan also binds and modulates the activity of a variety of non-matrix proteins, including BMP-4, C1q, collectins, TGF-beta, TNF-alpha, and WISP-1 (11, 12). Biglycan functions as a proinflammatory mediator by binding TLR2 and TLR4 on macrophages and inducing TNF-alpha and MIP-2 production (13). Biglycan knockout mice have compromised inflammatory responses and are resistant to LPS-induced septic shock (13). Biglycan binds HDL/Apolipoprotein E complexes in atherosclerotic plaques and also binds SR-A, blocking the uptake and degradation of LDL (14, 15).

References

Hocking, A.M. et al. (1998) Matrix Biol. 17:1.
Yoon, J.H. and J. Halper (2005) J. Musculoskelet. Neuronal Interact. 5:22.
Scott, I.C. et al. (2000) J. Biol. Chem. 275:30504.
Fisher, L.W. et al. (1989) J. Biol. Chem. 264:4571.
Scott, P.G. et al. (2006) J. Biol. Chem. 281:13324.
Schonherr, E. et al. (1995) J. Biol. Chem. 270:2776.
Wiberg, C. et al. (2003) J. Biol. Chem. 278:37698.
Reinboth, B. et al. (2006) J. Biol. Chem. 281:7816.
Roughley, P.J. et al. (1996) Biochem. J. 318:779.
Monfort, J. et al. (2006) Arthritis Res. Ther. 8:R26.
Groeneveld, T.W.L. et al. (2005) J. Immunol. 175:4715.
Moreno, M. et al. (2005) EMBO J. 24:1397.
Schaefer, L. et al. (2005) J. Clin. Invest. 115:2223.
Olin, K.L. et al. (2001) Arterioscler. Thromb. Vasc. Biol. 21:129.
Santiago-Garcia, J. et al. (2003) J. Biol. Chem. 278:6942.

Entrez Gene IDs

633 (Human); 12111 (Mouse)

Alternate Names

BGN; Biglycan; Bone/cartilage proteoglycan I; bone/cartilage proteoglycan-I; dermatan sulphate proteoglycan I; DSPG1; PGI; PG-S1; SLRR1A; SLRR1Abiglycan proteoglycan; small leucine-rich protein 1A

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Citations for Human Biglycan Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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