Human BLIMP1/PRDM1 Alexa Fluor® 647-conjugated Antibody Summary
Lys667-Cys789
Accession # O75626
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of BLIMP1/PRDM1 in U266 Human Cell Line by Flow Cytometry. U266 human myeloma cell line was stained with Mouse Anti-Human CD38 PE-conjugated Monoclonal Antibody (Catalog # FAB2404P) and either (A) Mouse Anti-Human BLIMP1/PRDM1 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # IC36081R) or (B) Mouse IgG1Alexa Fluor 647 Isotype Control (Catalog # IC002R). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
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Detection of BLIMP1/PRDM1 in Human PBMCs gated on CD19+CD3-cells by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) gated on CD19+CD3-cells were stained with Mouse Anti-Human BLIMP1/PRDM1 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # IC36081R) and Mouse Anti-Human CD38 PerCP-conjugated Monoclonal Antibody (Catalog # FAB2404C). Quadrant markers were set based on control antibody staining (Catalog # IC002R). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
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Detection of BLIMP1/PRDM1 in U266 cells by Flow Cytometry. U266 cells were stained with Mouse Anti-Human BLIMP1/PRDM1 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # IC36081R, filled histogram) or isotype control antibody (Catalog # IC002R, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: BLIMP1/PRDM1
Human BLIMP1 (B Lymphocyte-induced Maturation Protein 1), also known as PRDM1, is a 91 kDa zinc-finger transcriptional repressor that promotes B cell maturation into plasma cells. It is 789 amino acids (aa) in length, and contains an N-terminal S-E-T domain (aa 64-170) and four C-terminal C2H2-type zinc-finger motifs (aa 539‑645). The SET domain interacts with chromatin modifers, while the zinc fingers bind to DNA. There is one 80 kDa, 691 aa alternate splice form that utilizes an intenal start site. This results in a substitution of three aa for the first 101 aa of the long form, and the loss of the SET domain. At least 10 mutations exist, resulting in proteins of 61‑603 aa in length. Over aa 667-789, human BLIMP1 shares 89% aa sequence identity with mouse BLIMP1.
Product Datasheets
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
Citations for Human BLIMP1/PRDM1 Alexa Fluor® 647-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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B-cell targeting with anti-CD38 daratumumab: implications for differentiation and memory responses
Authors: Dorit Verhoeven, Lucas Grinwis, Casper Marsman, Machiel H Jansen, Ester MM T2B Consortium, Ester Mm Van Leeuwen et al.
Life Science Alliance
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MyD88/CD40 signaling retains CAR T cells in a less differentiated state
Authors: B Prinzing, P Schreiner, M Bell, Y Fan, G Krenciute, S Gottschalk
JCI Insight, 2020-11-05;5(21):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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