Human BLIMP1/PRDM1 APC-conjugated Antibody

Catalog #: IC36081A
4 Citations Datasheet / COA / SDS

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IC36081A

Detection of BLIMP1/PRDM1 in Human PBMCs by Flow Cytometry.

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All BLIMP1/PRDM1 Antibodies

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Citations (4)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BLIMP1/PRDM1 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human BLIMP1/PRDM1 in Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 646702

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human BLIMP1/PRDM1
Lys667-Cys789
Accession # O75626

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of BLIMP1/PRDM1 antibody in Human PBMCs antibody by Flow Cytometry.

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Detection of BLIMP1/PRDM1 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) gated on CD19⁺CD3^-cells were stained with Mouse Anti-Human BLIMP1/PRDM1 APC-conjugated Monoclonal Antibody (Catalog # IC36081A) and Mouse Anti-Human CD38 PerCP-conjugated Monoclonal Antibody (Catalog # FAB2404C). Quadrant markers were set based on control antibody staining (Catalog # IC0041P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry

Detection of BLIMP1/PRDM1 antibody in U266 Human Cell Line antibody by Flow Cytometry.

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Detection of BLIMP1/PRDM1 in U266 Human Cell Line by Flow Cytometry. U266 human myeloma cell line was stained with Mouse Anti-Human BLIMP1/PRDM1 APC-conjugated Monoclonal Antibody (Catalog # IC36081A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: BLIMP1/PRDM1

Human BLIMP1 (B lymphocyte-induced maturation protein 1, also known as PRDM1) is a 91 kDa zinc-finger transcriptional repressor that promotes B cell maturation into plasma cells. It is 789 amino acids (aa) in length, and contains an N-terminal S-E-T domain (aa 64-170) and four C-terminal C2H2-type zinc-finger motifs (aa 539‑645). The SET domain interacts with chromatin modifers, while the zinc fingers bind to DNA. There is one 80 kDa, 691 aa alternate splice form that utilizes an intenal start site. This results in a substitution of three aa for the first 101 aa of the long form, and the loss of the SET domain. At least 10 mutations exist, resulting in proteins of 61‑603 aa in length. Over aa 667-789, human BLIMP1 is 89% aa identical to mouse BLIMP1.

Long Name

B Lymphocyte Induced Maturation Protein

Entrez Gene IDs

639 (Human); 12142 (Mouse); 309871 (Rat)

Alternate Names

Beta-interferon gene positive regulatory domain I-binding factor; beta-interferon gene positive-regulatory domain I binding factor; BLIMP1; BLIMP-1; BLIMP1MGC118925; blmp-1; B-lymphocyte-induced maturation protein 1; MGC118922; MGC118923; Positive regulatory domain I-binding factor 1; PR domain containing 1, with ZNF domain; PR domain zinc finger protein 1; PR domain-containing protein 1; PRDI-BF1MGC118924; PRDI-binding factor 1; PRDI-binding factor-1; PRDM1; PR-domain zinc finger protein 1; ZNFPR1A1

Product Datasheets

Product Datasheet

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Citations for Human BLIMP1/PRDM1 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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PREX1 improves homeostatic proliferation to maintain a naïve CD4 T cell compartment in older age
Authors: Zhang, H;Okuyama, H;Jain, A;Jadhav, RR;Wu, B;Sturmlechner, I;Morales, J;Ohtsuki, S;Weyand, CM;Goronzy, JJ;
JCI insight
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Cholesterol metabolism drives regulatory B cell IL-10 through provision of geranylgeranyl pyrophosphate
Authors: JA Bibby, HA Purvis, T Hayday, A Chandra, K Okkenhaug, S Rosenzweig, I Aksentijev, M Wood, HJ Lachmann, C Kemper, AP Cope, E Perucha
Nat Commun, 2020-07-08;11(1):3412.
Species: Human
Sample Types: Cell Culture Lysates, Whole Cells
Applications: Flow Cytometry, Western Blot
Efficient CRISPR-Cas9-mediated mutagenesis in primary human B cells for identifying plasma cell regulators
Authors: Tuan Anh Le, Van Trung Chu, Andreia C. Lino, Eva Schrezenmeier, Christopher Kressler, Dania Hamo et al.
Molecular Therapy - Nucleic Acids
IL-21–Deficient T Follicular Helper Cells Support B Cell Responses Through IL-27 in Patients With Chronic Hepatitis B
Authors: Arshi Khanam, Natarajan Ayithan, Lydia Tang, Bhawna Poonia, Shyam Kottilil
Frontiers in Immunology