Human BMAL1 Antibody

Catalog #: MAB10560 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10560-100
MAB10560-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BMAL1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human BMAL1 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 1029536

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human BMAL1
Met105-Glu303
Accession # O00327

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human BMAL1 Monoclonal Antibody (Catalog # MAB105601).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human BMAL1 ELISA Standard Curve. Recombinant Human BMAL1 protein was serially diluted 2-fold and captured by Mouse Anti-Human BMAL1 Monoclonal Antibody (MAB105601) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human BMAL1 Monoclonal Antibody (Catalog # MAB10560) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMAL1

BMAL1 (Brain and Muscle ARNT-like 1; ARNTL) is an essential core component in circadian clock machinery which is regulatory mechanism for circadian rhythms. Circadian clock genes include three period proteins (PER1, PER2 and PER3), two cryptochromes (CRY1 and CRY2), CLOCK, NPAS2 and BMAL proteins, wherein BMAL1 has a potential to heterodimerize with CLOCK or NPAS2 genes; and this neocomplex drives transcription from E-box elements (5'-CACGTG-3') found in circadian-responsive genes's promoters. PER/CRY proteins negatively regulate CLOCK/BMAL1 dimer-mediated transcription, thereby forming the feedback loop that regulates the timing of clock gene transcription. BMAL1 also associates with GNB2L1/RACK1 and PRKCA in a nuclear complex, whertein GNB2L1 and PRKCA are recruited to the complex in a circadian manner. BMAL1 undergoes acetylation (Lys-538), phosphorylation and sumoylation (Lys-259) upon dimerization with CLOCK and acetylation facilitates CRY1-mediated repression. CLOCK-BMAL1 double mutations within PAS domains leads to syngernistic desensitization to high levels of CRY on repression of CLOCK-BMAL1 transcriptional activity of PER1 and, disrupt circadian rhythmicity. Clock genes functions primarily as tumor suppressors and their abbarant expression is observed in malignant pleural mesothelioma, colorectal and breast cancer.

Long Name

Brain and Muscle ARNT-Like 1

Entrez Gene IDs

406 (Human); 11865 (Mouse)

Alternate Names

ARNTL; aryl hydrocarbon receptor nuclear translocator-like protein 1; aryl hydrocarbon receptor nuclear translocator-like; basic-helix-loop-helix-PAS orphan MOP3; BHLHE5; bHLHe5brain and muscle; bHLH-PAS protein JAP3; BMAL1; BMAL1c; BMAL1TIC; Brain and muscle ARNT-like 1; Class E basic helix-loop-helix protein 5; JAP3; Member of PAS protein 3; member of PAS superfamily 3; MOP3; MOP3BMAL1c; PAS domain-containing protein 3; PASD3; PASD3MGC47515; TIC