Human BMP-15/GDF-9B Antibody

Catalog #: AF2925 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2925
AF2925-SP

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Human BMP-15/GDF-9B Antibody Summary

Species Reactivity

Human

Specificity

Detects human BMP-15/GDF-9B in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) BMP-2, rhBMP-3, rhBMP-4, rhBMP-5, rhBMP-6, rhBMP-7, rhBMP-8, and rhBMP-10 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human BMP-15/GDF-9B
Gln268-Arg392
Accession # O95972

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human BMP‑15/GDF‑9B

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-15/GDF-9B

Bone morphogenetic protein 15 (BMP-15), also known as GDF-9B, is a TGF-beta superfamily ligand that is expressed by oocytes throughout folliculogenesis, and plays an important role in oocyte development (1). BMP-15 promotes the FSH-independent proliferation of ovarian granulosa cells (GC) and induces GC glycolysis and cholesterol synthesis (2‑4). It also induces GC production of stem cell factor which, in turn, negatively regulates BMP-15 expression in oocytes (5). BMP-15 blocks the FSH-induced GC expression of FSH R and multiple steroidogenic molecules (6). BMP-15 is synthesized with a 249 amino acid (aa) N-terminal propeptide (7). The propeptide is cleaved intracellularly from the 50 kDa proBMP-15 but remains associated with mature BMP-15 (8). Mature BMP-15 exists in 16 kDa and 17 kDa forms which are distinguishable by the presence of O-linked glycosylation on the 17 kDa form (8). Mature BMP-15 is phosphorylated, a modification which is required for the stimulation of GC proliferation (9). BMP-15 exerts its effects through interactions with BMPR-IB/ALK6 and BMPR-II (9‑11). Mature BMP-15 forms 34 kDa noncovalently-linked homodimers and 37 kDa heterodimers with mature GDF-9 (12). Both of these oocyte-expressed factors lack the cysteine that mediates disulfide-linked dimerization in most TGF-beta superfamily ligands (1). Although heterodimerization with GDF-9 may limit the secretion of active BMP-15, these two factors synergize in promoting oocyte survival and folliculogenesis (12, 13). Mature human BMP-15 shares 70%, 68%, and 78% aa sequence identity with mouse, rat, and sheep BMP-15, respectively. It shares 27%‑38% aa sequence with other BMPs.

References

Moore, R.K. and S. Shimasaki (2005) Mol. Cell. Endocrinol. 234:67.
Otsuka, F. et al. (2000) J. Biol. Chem. 275:39523.
Sugiura, K. et al. (2007) Development 134:2593.
Su, Y.-Q. et al. (2008) Development 135:111.
Otsuka, F. and S. Shimasaki (2002) Proc. Natl. Acad. Sci. 99:8060.
Otsuka, F. et al. (2001) J. Biol. Chem. 276:11387.
Dube, J.L. et al. (1998) Mol. Endocrinol. 12:1809.
Saito, S. et al. (2008) Prot. Sci. 17:362.
McMahon, H.E. et al. (2008) Endocrinology 149:812.
Moore, R.K. et al. (2003) J. Biol. Chem. 278:304.
Edwards, S.J. et al. (2008) Endocrinology 149:1026.
Liao, W.X. et al. (2003) J. Biol. Chem. 278:3713.
Yan, C. et al. (2001) Mol. Endocrinol. 15:854.

Long Name

Bone Morphogenetic Protein 15

Entrez Gene IDs

9210 (Human); 12155 (Mouse); 59302 (Rat)

Alternate Names

BMP15; BMP-15; bone morphogenetic protein 15; GDF-9B; Growth/differentiation factor 9B; ODG2; POF4

Product Datasheets

Product Datasheet

COA

SDS

Product Specific Notices

This product is sold under license from Vanderbilt University for research and laboratory use only. Licenses for commercial manufacture, commercial use, diagnostics or therapeutics may beobtained by contacting Vanderbilt University, Nashville, Tennessee.