Human BMP-3 Antibody

Catalog #: AF113 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF113
AF113-SP

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Human BMP-3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human BMP-3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) BMP‑2, rhBMP-4, rhBMP-5, rhBMP-6, and rhBMP-7 is observed. This antibody very weakly recognizes native dimeric BMP-3.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human BMP‑3
Ser346-Arg472
Accession # P12645

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human BMP‑3 (Catalog # 113-BP)

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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BMP‑3 in Human Prostate Cancer Tissue. BMP‑3 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using 15 µg/mL Goat Anti-Human BMP‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF113) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-3

BMP-3, also known as osteogenin, the most abundant BMP in adult bone, is one of at least 15 structurally and functionally related BMPs, which are members of the TGF- beta superfamily (1‑3). BMPs were originally identified as protein regulators of cartilage and bone formation. They have since been shown to be involved in embryogenesis and morphogenesis of various tissues and organs. BMPs also regulate the growth, differentiation, chemotaxis, and apoptosis of various cell types. Similar to most other TGF-beta family proteins, BMPs are highly conserved across animal species. At the amino acid sequence level, mature human and rat BMP-3 are 98% identical. BMP-3 is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. Biologically active BMP-3 is a disulfide-linked homodimer of the carboxy-terminal 110 amino acid residues that contains the characteristic seven conserved cysteine residues involved in the formation of the cysteine knot and the single interchain disulfide bond (4). The role of BMP-3 in bone is contradictory since, unlike osteogenin purified from bone, recombinant BMP-3 has not shown osteogenic function (5). Several studies indicate that BMP-3 is an inhibitor of osteogenic BMPs. BMP-3 dorsalizes Xenopus embryos, the opposite effect of BMP-2 or 4, which cause ventralization. BMP-3 inhibits alkaline phosphatase production and induction of osteoblastic target genes in undifferentiated mesenchymal and osteogenic cell lines that have been treated with BMP-2. BMP-3 also induces the expression of TGF‑ beta /activin responsive genes, but not BMP-responsive genes. Since the inhibitory effect is not due to direct competition with osteogenic BMPs, it has been suggested that BMP-3 activates signaling through an activin pathway, resulting in antagonism of osteogenesis induced by other BMPs.

References

Chen, D. et al. (2004) Growth Factors 22:233.
Hino, J. et al. (2004) Front. Biosci. 9:1520.
Bahamonde, M.E. and K.M. Lyons (2001) J. Bone and Joint Surgery 83-A (suppl 1):S156.
Wozney, J.M. et al. (1998) Science 242:1528.
Daluiski, A. et al. (2001) Nature Genetics 27:84.

Long Name

Bone Morphogenetic Protein 3

Entrez Gene IDs

651 (Human); 110075 (Mouse); 25667 (Rat)

Alternate Names

BMP3; BMP-3; BMP3A; BMP-3A; bone morphogenetic protein 3 (osteogenic); bone morphogenetic protein 3; Bone morphogenetic protein 3A; bone morphogenetic protein-3; Osteogenin