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Human BMP-7 Antibody

Catalog #: MAB3542
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3542-SP
MAB3542-100
MAB3542-500
Alkaline Phosphatase Production Induced by BMP‑7 and Neutralization by Human BMP‑7 Antibody.
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Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BMP-7 Antibody Summary

Species Reactivity
Human
Specificity
Detects human BMP-7 in ELISAs. In ELISAs, this antibody shows approximately 25-50% cross-reactivity with recombinant human (rh) BMP‑6 and no cross-reactivity with rhBMP-2, -3, -4, -5, or -8.
Source
Monoclonal Mouse IgG1 Clone # 164313
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human BMP‑7
Arg292-His431
Accession # P18075
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Human BMP-7 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human BMP‑7 Biotinylated Antibody (Catalog # BAM354)

Standard: Recombinant Human BMP-7 Protein (Catalog # 354-BP)

Neutralization
Measured by its ability to neutralize BMP‑7-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. Erlacher, L. et al. (1998) J. Bone Miner. Res. 13:383. The Neutralization Dose (ND50) is typically 4-12 µg/mL in the presence of 1 µg/mL Recombinant Human BMP‑7 and 50 µg/mL L-ascorbic acid.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Alkaline Phosphatase Production Induced by BMP‑7 and Neutralization by Human BMP‑7 Antibody. View Larger

Alkaline Phosphatase Production Induced by BMP‑7 and Neutralization by Human BMP‑7 Antibody. Recombinant Human BMP‑7 (Catalog # 354-BP) induces alkaline phosphatase production in the the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP‑7 (1 µg/mL) is neutralized (green line) by increasing concentrations of Human BMP‑7 Monoclonal Antibody (Catalog # MAB3542). The ND50 is typically 4-12 µg/mL in the presence of L-ascorbic acid (50 µg/mL).

Immunohistochemistry BMP‑7 antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

BMP‑7 in Human Prostate Cancer Tissue. BMP‑7 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 25 µg/mL Human BMP‑7 Monoclonal Antibody (Catalog # MAB3542) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry BMP-7 antibody in Human Prostate by Immunohistochemistry (IHC-P). View Larger

BMP‑7 in Human Prostate. BMP-7 was detected in immersion fixed paraffin-embedded sections of human prostate using Human BMP-7 Monoclonal Antibody (Catalog # MAB3542) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-7

BMP-7, or osteogenic protein 1 (OP-1), is one of at least 15 structurally and functionally related BMPs, which are members of the transforming growth factor beta (TGF-beta ) superfamily. BMPs regulate cartilage and bone formation, embryogenesis and morphogenesis of various tissues and organs, and growth, differentiation, chemotaxis and apoptosis of various cell types. Biologically active BMP-7 is a disulfide-linked homodimer. Cellular responses to BMP-7 have been shown to be mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors.

Long Name
Bone Morphogenetic Protein 7
Entrez Gene IDs
655 (Human); 12162 (Mouse); 85272 (Rat)
Alternate Names
BMP7; BMP-7; bone morphogenetic protein 7; Eptotermin alfa; OP-1; OP-1OP1; Osteogenic protein 1

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Citations for Human BMP-7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. BMP7 Expression Correlates with Secondary Drug Resistance in Mantle Cell Lymphoma
    Authors: Valérie Camara-Clayette, Serge Koscielny, Sébastien Roux, Thierry Lamy, Jacques Bosq, Marc Bernard et al.
    PLoS ONE
  2. Expression of bone morphogenetic protein 2, 4, 5, and 7 correlates with histological activity of otosclerotic foci
    Authors: Péter Csomor, Balázs Liktor, Bálint Liktor, Zoltán Szekanecz, István Sziklai, Tamás Karosi
    Acta Oto-Laryngologica
  3. Extracellular Regulation of Bone Morphogenetic Protein Activity by the Microfibril Component Fibrillin-1.
    Authors: Wohl A, Troilo H, Collins R, Baldock C, Sengle G
    J Biol Chem, 2016-04-08;291(24):12732-46.
  4. Functional Characterization of Two New Variants in the Bone Morphogenetic Protein 7 Prodomain in Two Pairs of Monozygotic Twins With Hypospadias
    Authors: Aurore Bouty, Kelly Walton, Nurin Aisyiyah Listyasari, Gorjana Robevska, Jocelyn Van den Bergen, Ardy Santosa et al.
    Journal of the Endocrine Society
  5. The anti-M�llerian hormone prodomain is displaced from the hormone/prodomain complex upon bivalent binding to the hormone receptor
    Authors: RL Cate, N di Clement, C Racine, NP Groome, RB Pepinsky, A Whitty
    The Journal of Biological Chemistry, 2021-11-19;0(0):101429.
    Species: Human
    Sample Types: Recombinant Proteins
    Applications: ELISA Detection
  6. Bone morphogenetic protein-6 promotes osteoblastic prostate cancer bone metastases through a dual mechanism.
    Authors: Dai J, Keller J, Zhang J, Lu Y, Yao Z, Keller ET
    Cancer Res., 2005-09-15;65(18):8274-85.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Activation of TGF-beta Pathway by Areca Nut Constituents: A Possible Cause of Oral Submucous Fibrosis
    Authors: Imran Khan, Neeraj Kumar, Ila Pant, Sivakrishna Narra, Paturu Kondaiah
    PLoS ONE
  8. Micellized Protein Transduction Domain-Bone Morphogenetic Protein-7 Efficiently Blocks Renal Fibrosis Via Inhibition of Transforming Growth Factor-Beta–Mediated Epithelial–Mesenchymal Transition
    Authors: Seonghun Kim, Cheol-Hee Jeong, Sang Hyun Song, Jo Eun Um, Hyun Sil Kim, Jun Seop Yun et al.
    Frontiers in Pharmacology

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