Human BMP-8 Antibody

Catalog #: MAB1073 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1073
MAB1073-SP

All BMP-8 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BMP-8 Antibody Summary

Species Reactivity

Human

Specificity

Detects human BMP-8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human BMP-2, -3, -4, -5, -6, or -7 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 158708

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human BMP‑8
Ala264-His402
Accession # AAP74559

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human BMP‑8

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-8

BMP-8, also known as osteogenic protein 2 (OP-2), was first isolated from a hippocampal library in a screen to identify relatives of BMP-7/OP-1 (1). BMPs are a family of structurally and functionally related proteins and represent a subfamily of the transforming growth factor beta (TGF-beta ) superfamily. BMPs are involved in a wide range of processes including embryogenesis, tissue morphogenesis, cell differentiation and migration, and tumorigenesis. Cellular responses to BMPs are mediated by hetero‑oligomeric complexes of type I and type II serine/threonine kinase receptors (2‑4). BMP‑8a and BMP-8b, produced from separate genes, share 98% aa sequence identity in human but only 74% in mouse within the mature regions. Human BMP‑8a is synthesized as a 402 aa precursor protein that is cleaved between Arg263 and Ala264 to release the C-terminal mature protein. Mature human BMP‑8a shares 90% and 68% aa sequence identity with mouse mature BMP‑8a and -8b, respectively. BMP‑8a is expressed during pregnancy in the deciduum and trophoblast cells, by inner root sheath cells of developing hair follicles, and by the epididymis and spermatids (5‑7). In the mouse it cooperates with BMP-7 in the maintenance of spermatogenesis but is not required for the initiation of spermatogenesis (7, 8). BMP‑8b, in contrast, is required for both the initiation and maintenance of spermatogenesis (9). BMP‑8a is induced during osteoblast differentiation at the onset of mineralization and during the osteogenic phase of bone repair in osteoblasts and osteocytes (10‑12). BMP‑8a/b is also highly expressed in osteosarcomas (13).

References