Human BMPR-IB/ALK-6 Antibody

Catalog #: AF505
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF505
AF505-SP

Product Details

Citations (4)

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Human BMPR-IB/ALK-6 Antibody Summary

Species Reactivity

Human

Specificity

Detects human BMPR-IB in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse BMPR‑IB is observed and less than 1% cross-reactivity with recombinant human (rh) BMPR-IA and rhBMPR-II is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human BMPR-IB/ALK-6
Lys14-Arg126
Accession # O00238

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human BMPR-IB/ALK‑6 Fc Chimera (Catalog # 505-PR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMPR-IB/ALK-6

Cellular responses to bone morphogenetic proteins (BMPs) have been shown to be mediated by the formation of hetero-oligomeric complexes of the type I and type II serine/threonine kinase receptors. BMP receptor IB (BMPR-IB), also known as activin receptor-like kinase (ALK)-6, is a one of seven known type I serine/threonine kinases that are required for the signal transduction of TGF-beta family cytokines. In contrast to the TGF-beta receptor system in which the type I receptor does not bind TGF-beta in the absence of the type II receptor, type I receptors involved in BMP signaling (including BMPR-IA, BMPR-IB/ALK-6, and ActR-I/ALK-2) can independently bind the various BMP family proteins in the absence of type II receptors. Recombinant soluble BMPR-IB binds BMP-4 with high-affinity in solution and is a potent BMP-4 antagonist in vitro. BMPR-IB is expressed in various tissues during embryogenesis. In adult tissues, BMPR-IB is only found in the brain. The extracellular domain of BMPR-IB shares little amino acid sequence identity with the other mammalian ALK type I receptor kinases, but the cysteine residues are conserved. Human and mouse BMPR-IB are highly conserved and share 98% sequence identity.

References

Kawabata, M. et al. (1998) Cytokine and Growth Factor Reviews 9:49.
Ebendal, T. et al. (1998) J. Neuroscience Research 51:139.

Long Name

Bone Morphogenetic Protein Receptor IB

Entrez Gene IDs

658 (Human); 12167 (Mouse)

Alternate Names

activin receptor-like kinase 6; ALK-6; ALK6CDw293; BMP type-1B receptor; BMPR1B; BMPR-1B; BMPRIB; BMPR-IB; bone morphogenetic protein receptor type-1B; bone morphogenetic protein receptor, type IB; CDw293 antigen; CDw293; EC 2.7.11; EC 2.7.11.30; serine/threonine receptor kinase

Product Datasheets

Product Datasheet

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SDS

Related Research Areas

Citations for Human BMPR-IB/ALK-6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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BMP4 inhibits breast cancer metastasis by blocking myeloid-derived suppressor cell activity.
Authors: Cao Y, Slaney C, Bidwell B, Parker B, Johnstone C, Rautela J, Eckhardt B, Anderson R
Cancer Res, 2014-09-15;74(18):5091-102.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Bone morphogenetic protein-9 activates Smad and ERK pathways and supports human osteoclast function and survival in vitro.
Authors: Fong D, Bisson M, Laberge G, McManus S, Grenier G, Faucheux N, Roux S
Cell Signal, 2013-01-08;25(4):717-28.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot
Epigenetic-mediated dysfunction of the bone morphogenetic protein pathway inhibits differentiation of glioblastoma-initiating cells.
Authors: Lee J, Son MJ, Woolard K, Donin NM, Li A, Cheng CH, Kotliarova S, Kotliarov Y, Walling J, Ahn S, Kim M, Totonchy M, Cusack T, Ene C, Ma H, Su Q, Zenklusen JC, Zhang W, Maric D, Fine HA
Cancer Cell, 2008-01-01;13(1):69-80.
Species: Human, Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Sequential roles for myosin-X in BMP6-dependent filopodial extension, migration, and activation of BMP receptors.
Authors: Pi X, Ren R, Kelley R, Zhang C, Moser M, Bohil AB, Divito M, Cheney RE, Patterson C
J. Cell Biol., 2007-12-24;179(7):1569-82.
Species: Mouse
Sample Types: Cell Lysates
Applications: Immunoprecipitation, Western Blot