Human BMPR-IB/ALK-6 APC-conjugated Antibody

Catalog #: FAB5051A
3 Citations Datasheet / COA / SDS

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FAB5051A

Detection of BMPR‑IB/ALK‑6 in PC‑3 Human Cell Line by Flow Cytometry.

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Human BMPR-IB/ALK-6 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human BMPR‑IB/ALK‑6 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse BMPR-IB is observed.

Source

Monoclonal Mouse IgG_2B Clone # 477914

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human BMPR‑IB/ALK‑6
Lys14-Arg126
Accession # O00238

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of BMPR‑IB/ALK‑6 in PC‑3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-Human BMPR-IB/ALK-6 APC-conjugated Monoclonal Antibody (Catalog # FAB5051A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of BMPR‑IB/ALK‑6 in Human iPS cells differentiated to Mesoderm by Flow Cytometry. Human iPS cells differentiated to mesoderm (using Catalog # SC030B) were stained with Mouse Anti-Human BMPR-IB/ALK-6 APC-conjugated Monoclonal Antibody (Catalog # FAB5051A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: BMPR-IB/ALK-6

Cellular responses to bone morphogenetic proteins (BMPs) have been shown to be mediated by the formation of hetero-oligomeric complexes of the type I and type II serine/threonine kinase receptors. BMP receptor IB (BMPR-IB), also known as activin receptor-like kinase (ALK)-6, is one of seven known type I serine/threonine kinases that are required for the signal transduction of TGF-beta family cytokines. In contrast to the TGF-beta receptor system in which the type I receptor does not bind TGF-beta in the absence of the type II receptor, type I receptors involved in BMP signaling (including BMPR-IA, BMPR-IB/ALK-6, and ActR-I/ALK-2) can independently bind the various BMP family proteins in the absence of type II receptors. Recombinant soluble BMPR-IB binds BMP-4 with high-affinity in solution and is a potent BMP-4 antagonist in vitro. BMPR-IB is expressed in various tissues during embryogenesis. In adult tissues, BMPR-IB is only found in the brain. The extracellular domain of BMPR-IB shares little amino acid sequence identity with the other mammalian ALK type I receptor kinases, but the cysteine residues are conserved. Human and mouse BMPR-IB are highly conserved and share 98% amino acid sequence identity.

References

Kawabata, M. et al. (1998) Cytokine and Growth Factor Reviews 9:49.
Ebendal, T. et al. (1998) J. Neuroscience Research 51:139.

Long Name

Bone Morphogenetic Protein Receptor IB

Entrez Gene IDs

658 (Human); 12167 (Mouse)

Alternate Names

activin receptor-like kinase 6; ALK-6; ALK6CDw293; BMP type-1B receptor; BMPR1B; BMPR-1B; BMPRIB; BMPR-IB; bone morphogenetic protein receptor type-1B; bone morphogenetic protein receptor, type IB; CDw293 antigen; CDw293; EC 2.7.11; EC 2.7.11.30; serine/threonine receptor kinase

Product Datasheets

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Citations for Human BMPR-IB/ALK-6 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Osteogenic differentiation of human mesenchymal stromal cells and fibroblasts differs depending on tissue origin and replicative senescence
Authors: V Grotheer, N Skrynecki, L Oezel, J Grassmann, J Windolf, CV Suschek
Scientific Reports, 2021-06-07;11(1):11968.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Chronic myeloid leukaemia cells require the bone morphogenic protein pathway for cell cycle progression and self-renewal
Authors: P Toofan, C Busch, H Morrison, S O'Brien, H Jørgensen, M Copland, H Wheadon
Cell Death Dis, 2018-09-11;9(9):927.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Mapping molecular landmarks of human skeletal ontogeny and pluripotent stem cell-derived articular chondrocytes
Authors: GB Ferguson, B Van Handel, M Bay, P Fiziev, T Org, S Lee, R Shkhyan, NW Banks, M Scheinberg, L Wu, B Saitta, J Elphingsto, AN Larson, SM Riester, AD Pyle, NM Bernthal, HK Mikkola, J Ernst, AJ van Wijnen, M Bonaguidi, D Evseenko
Nat Commun, 2018-09-07;9(1):3634.
Species: Human
Sample Types: Whole Cells
Applications: Cell Selection