Human BMX Antibody

Catalog #: AF5887 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5887
AF5887-SP

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Human BMX Antibody Summary

Species Reactivity

Human

Specificity

Detects human BMX in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human BMX
Met1-Gln215
Accession # P51813

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human BMX by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Human BMX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5887) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BMX at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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BMX in Human Prostate. BMX was detected in immersion fixed paraffin-embedded sections of human prostate using Human BMX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5887) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMX

BMX (Bone marrow tyrosine kinase gene in chromosome X protein; also ETK and NTK38) is a 76‑85 kDa member of the TEC subfamily, Tyr protein kinase family of enzymes. It is a cytoplasmic endothelial and epithelial cell kinase that induces STAT phosphorylation in a Jak-independent manner, and regulates cytokine production via mRNA stabilization. Human BMX is 675 amino acids (aa) in length. It contains one PH domain whose ligation induces activation (aa 6‑109), a zinc-finger region (aa 113‑149), two autophosphorylation sites at Tyr216 and Tyr224, an SH3 and SH2 domain (aa 213‑392) and a kinase catalytic site (aa 417‑675). BMX undergoes proteolysis by caspase 3 to generate a 35 kDa N-terminal (aa 1‑242) fragment, and a 50 kDa C-terminal (aa 243‑675) fragment with enhanced kinase activity.

Long Name

BMX Non-receptor Tyrosine Kinase

Entrez Gene IDs

660 (Human); 12169 (Mouse); 367786 (Rat)

Alternate Names

BMX non-receptor tyrosine kinase; BMX; Bone marrow tyrosine kinase gene in chromosome X protein; EC 2.7.10; EC 2.7.10.2; Epithelial and endothelial tyrosine kinase; ETK; Etk/Bmx cytosolic tyrosine kinase; ETKcytoplasmic tyrosine-protein kinase BMX; NTK38 tyrosine kinase; NTK38; PSCTK2; PSCTK3