Human BNIP3 Antibody Summary
Ser2-Glu160
Accession # Q12983
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human BNIP3 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 150 µM CoCl2for 18 hours. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human BNIP3 Monoclonal Antibody (Catalog # MAB4147) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for BNIP3 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BNIP3
Bcl-2/adenovirus E1B 19 kDa protein-interacting protein 3 (BNIP3), also known as 19 kDa interacting protein 3 (NIP3), is a proapoptotic member of Bcl-2 protein family. BNIP3 is a 194 amino acid, 21.5 kDa (predicted) protein that contains a single Bcl-2 homology 3 (BH3) domain and a C-terminal transmembrane domain required for mitochondrial localization, homodimerization, and regulation of its proapoptotic function. BNIP3 was identified as one of several proteins that interact with discrete domains of Bcl-2 and the E1B 19 kDa protein. Under conditions of prolonged oxygen deprivation, the hypoxia-induced protein HIF1-alpha activates expression of BNIP3, which in turn, promotes apoptosis under these conditions. The mechanism of BNIP3-mediated apoptosis is independent of caspase activation and cytochrome c release and is characterized by early plasma membrane and mitochondrial damage, prior to the appearance of chromatin condensation or DNA fragmentation. Human BNIP3 shares 90% amino acid identity with mouse and rat BNIP3. Human BNIP3 shares 56% amino acid sequence identity with human BNIP3L.
Product Datasheets
Citations for Human BNIP3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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High content screening identifies monensin as an EMT-selective cytotoxic compound
Authors: M Vanneste, Q Huang, M Li, D Moose, L Zhao, MA Stamnes, M Schultz, M Wu, MD Henry
Sci Rep, 2019-02-04;9(1):1200.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Autophagy Induction Results in Enhanced Anoikis-resistance in Models of Peritoneal Disease
Authors: John Hays
Mol. Cancer Res., 2016-11-02;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Hypoxia counteracts taxol-induced apoptosis in MDA-MB-231 breast cancer cells: role of autophagy and JNK activation.
Authors: Notte A, Ninane N, Arnould T, Michiels C.
Cell Death Dis.
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