Human Bora Antibody

Catalog #: MAB68561 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB68561
MAB68561-SP

Detection of Human Bora by Western Blot.

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Human Bora Antibody Summary

Species Reactivity

Human

Specificity

Detects human Bora in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 694746

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Bora
Gly2-Ala180
Accession # Q6PGQ7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Bora by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Bora Monoclonal Antibody (Catalog # MAB68561) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Bora at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Bora

Bora (Aurora borealis; also C13orf34) is a 61 kDa member of the Bora family of proteins. It is ubiquitously expressed, and plays a key role in cell cycle progression. Plk1 (polo-like kinase-1) is a phosphorylase that is important to the cell during the G2/M transition and mitosis. Its activity is initially regulated by Aurora-A, which phosphorylates and activates Plk1 on Thr210. Bora, Aurora-A and Plk1 all appear to form a complex during G2. Bora predisposes Plk1 to the actions of Aurora-A. Once activated by Aurora-A, Plk1 drives the mitotic mechanism, which includes a third-party phosphorylation of Bora. This initiates BORA dissociation from Aurora-A with subsequent ubiquitination and degradation. Human Bora is 559 amino acids (aa) in length. It contains a Ser-rich region (aa 188-278) and at least eight utilized Ser phosphorylation sites. Phosphorylation may increase the SDS-PAGE MW of Bora to 75-85 kDa. There is one potential alternative start site that lies 60 aa upstream of the standard start site, and a second splice variant the shows a 17 aa substitution for aa 1-87. Over aa 2-180, human Bora shares 84% aa identity with mouse Bora.

Long Name

Protein Aurora Borealis

Entrez Gene IDs

79866 (Human); 777441 (Mouse); 306102 (Rat)

Alternate Names

Aurora Borealis; Bora; C13orf34; chromosome 13 open reading frame 34; FLJ22624; hsBora; protein aurora borealis