Human BTN3A1/2/3 APC-conjugated Antibody

Catalog #: FAB7136A Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
FAB7136A-025

Detection of BTN3A1/2/3 in Human Blood Lymphocytes by Flow Cytometry.

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Human BTN3A1/2/3 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human BTN3A1, BTN3A2 and BTN3A3 in direct ELISAs.

Source

Recombinant Monoclonal Mouse IgG₁ Clone # 849203

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human CD277/BTN3A1
Met1-Gly254
Accession # O00481

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of BTN3A1/2/3 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human BTN3A1/2/3 APC-conjugated Monoclonal Antibody (Catalog # FAB7136A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: BTN3A1/2/3

Butyrophilin BTN3A1, also known as CD277, has high structural homology to the B7 superfamily of proteins and is expressed in various immune cells such as T and NK cells. BTN3A1 enhances TCR-induced cytokine production and cell proliferation. Early T-cell activation events such as TCR-induced cell signaling are increased upon BTN3A1 engagement. The BTN3A1 co-stimulatory pathway may be involved in the regulation of various cell-mediated immune responses.

Long Name

Butyrophilin, Subfamily 3, Member A1/2/3

Alternate Names

BT3.1; BTF5; BTN3.1; BTN3A1/2/3; butyrophilin subfamily 3 member A1; CD277; dJ45P21.3 (butyrophilin, subfamily 3, member A1)