Human c-Jun Antibody

Catalog #: AF2670
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2670
AF2670-SP

Detection of Human c-Jun by Western Blot.

1 Image

All c-Jun Antibodies

Product Details

Citations (1)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human c-Jun Antibody Summary

Species Reactivity

Human

Specificity

Detects human c-Jun in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human c-Jun
Thr2-Phe331
Accession # P05412

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human c-Jun by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, and MDA-MB-468 human breast cancer cell lines. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human c-Jun Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2670) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for c-Jun at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: c-Jun

c-Jun is one of three Jun family proteins making up the Activator Protein-1 (AP-1) transcription factor group. c-Jun acts as a positive regulator of cell proliferation and can induce oncogenic transformation.

Long Name

Cellular Repressor of E1A-stimulated Genes/Transcription Factor AP-1

Entrez Gene IDs

3725 (Human)

Alternate Names

Activator protein 1; AP1; AP-1; cJun; c-Jun; enhancer-binding protein AP1; Jun activation domain binding protein; jun oncogene; jun proto-oncogene; JUN; Proto-oncogene c-Jun; transcription factor AP-1; V-jun avian sarcoma virus 17 oncogene homologp39; v-jun sarcoma virus 17 oncogene homolog (avian); v-jun sarcoma virus 17 oncogene homolog

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human c-Jun Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1