Human C10orf99 Antibody

Catalog #: MAB10416 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10416-100
MAB10416-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human C10orf99 Antibody Summary

Species Reactivity

Human

Specificity

Detects human C10orf99 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 1020903

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived human C10orf99

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human C10orf99 Monoclonal Antibody (Catalog # MAB104161).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human C10orf99 ELISA Standard Curve. Recombinant Human C10orf99 protein was serially diluted 2-fold and captured by Mouse Anti-Human C10orf99 Monoclonal Antibody (Catalog # MAB10416) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human C10orf99 Monoclonal Antibody (Catalog # MAB104161) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: C10orf99

Human Chromosome 10 open reading frame 99 is a 9.2 KDa protein encoded by the C10orf99 gene. C10orf99 has been described as a potential cytokine and a ligand Sushi Domain Containing 2 (SUSD2), inducing SUSD2 internalization upon binding. It has also been proposed to be a chemotactic factor that mediates lymphocytes recruitment to epithelia through binding and activation of the G-protein coupled receptor GPR15.

Long Name

Chromosome 10 Open Reading Frame 99

Entrez Gene IDs

387695 (Human); 70045 (Mouse)

Alternate Names

AP57; AP-57; C10orf99 chromosome 10 open reading frame 99; C10orf99; GPR15L; UNQ1833