Human Calcitonin R Antibody

Catalog #: MAB46141 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB46141-100
MAB46141-SP

Detection of Human Calcitonin R by Western Blot.

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All Calcitonin R Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Calcitonin R Antibody Summary

Species Reactivity

Human

Specificity

Detects human Calcitonin R in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 462815

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

HEK293 human embryonic kidney cell line transfected with human Calcitonin R
Ala25-Ala474
Accession # NP_001733

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunohistochemistry

5-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Calcitonin R by Western Blot. Western blot shows lysates of HEK293T human embryonic kidney cell line mock transfected or tranfected with Calcitonin R. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Calcitonin R Monoclonal Antibody (Catalog # MAB46141) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Calcitonin R at approximately 90-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Calcitonin R in Human Pancreatic Cancer Tissue. Calcitonin R was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Mouse Anti-Human Calcitonin R Monoclonal Antibody (Catalog # MAB46141) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Calcitonin R

Calcitonin receptor (CALCR) is a glycosylated 70 kDa seven-transmembrane G protein-coupled receptor that mediates the hypocalcemic effects of the peptide hormone, calcitonin. CALCR activation inhibits osteoclast-mediated bone resorption and enhances renal calcium excretion. CALCR polymorphisms and mutations have been associated with several bone disorders. Alternative splicing results in the deletion of 16 aa in the first cytoplasmic loop or 23 aa in the first extracellular region. Human CALCR shares 70%‑72% aa sequence identity with mouse and rat CACLR.

Long Name

Calcitonin Receptor

Entrez Gene IDs

799 (Human); 12311 (Mouse); 116506 (Rat)

Alternate Names

Calcitonin R; calcitonin receptor; CalcitoninR; CALCR; CRT; CTR; CT-R; CTR1