Human Calreticulin APC-conjugated Antibody
Human Calreticulin APC-conjugated Antibody Summary
Met1-Asn180
Accession # P27797
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Calreticulin in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human Calreticulin APC-conjugated Monoclonal Antibody (Catalog # IC3898A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). Cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
Background: Calreticulin
Human Calreticulin is a 55‑60 kDa, 400 amino acid, variably glycosylated intra- and extracellular Ca++-binding lectin that is ubiquitously expressed. It consists of three domains: a 180 aa N-terminal globular region, a 111 aa P-, or proline rich domain, and a 109 aa C-terminus. The 180 aa N-terminus (aa 18‑197) is termed Vasostatin. It is unclear if it is ever generated naturally via proteolytic processing. Vasostatin domain has many functions. It binds to RNA (aa 18‑27), has autocatalytic phosphorylase activity (aa 77‑197), binds to a KxFFKR motif on steroid hormone receptors, and serves as a lectin-type chaperone for ER-localized molecules. It also shows antiangiogenic activity, presumably by binding to laminin carbohydrates and blocking endothelial cell adhesion and proliferation. Human Calreticulin is 94% aa identical to mouse and rat Calreticulin.
Product Datasheets
Citation for Human Calreticulin APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Perturbation-Based Modeling Unveils the Autophagic Modulation of Chemosensitivity and Immunogenicity in Breast Cancer Cells
Authors: I Quiros-Fer, L Figueroa-P, JL Arias-Aria, N Brenes-Cor, F Siles, J Mora, RA Mora-Rodrí
Metabolites, 2021-09-18;11(9):.
Species: Human
Sample Types: Transfected Whole Cells
Applications: Flow Cytometry
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