Human Cannabinoid R1/CB1/CNR1 Antibody

Catalog #: MAB3834 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3834
MAB3834-SP

Detection of Cannabinoid R1/CB1/CNR1 in NS0 Mouse Cell Line Transfected with Human Cannabinoid R1/CB1/CNR1 and eGFP by Flow Cytometry.

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Human Cannabinoid R1/CB1/CNR1 Antibody Summary

Species Reactivity

Human

Specificity

Stains Cannabinoid R1/CB1/CNR1-transfected cells but not irrelevant transfectants.

Source

Monoclonal Mouse IgG_2A Clone # 368302

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

HEK293 human embryonic kidney cell line transfected with human Cannabinoid R1/CB1/CNR1
Met1-Leu472
Accession # NP_057167

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

Detection of Cannabinoid R1/CB1/CNR1 antibody in NS0 Mouse Cell Line Transfected with Human Cannabinoid R1/CB1/CNR1 and eGFP antibody by Flow Cytometry.

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Detection of Cannabinoid R1/CB1/CNR1 in NS0 Mouse Cell Line Transfected with Human Cannabinoid R1/CB1/CNR1 and eGFP by Flow Cytometry. NS0 mouse myeloma cell line transfected with human Cannabinoid R1/CB1/CNR1 and eGFP was stained with and either (A) Mouse Anti-Human Cannabinoid R1/CB1/CNR1 Monoclonal Antibody (Catalog # MAB3834) or (B) Mouse IgG_2AIsotype Control (Catalog # MAB003) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cannabinoid R1/CB1/CNR1

Cannabinoid R1 (CB1R) is a 60 kDa 7TM protein that belongs to the family of G protein-coupled receptors, class A. CB1R is expressed in the central nervous system and upper GI tract, in contrast to CB2R which is expressed by hematopoietic cells. CB1R mediates the behavioral and gut motility effects of cannabinoids. Human CB1R exists in three alternately spliced forms which are distinguished by N-terminal substitutions or deletions. Human CB1R shares 97% amino acid sequence identity with mouse and rat CB1R.

Long Name

Cannabinoid Receptor 1

Entrez Gene IDs

1268 (Human); 12801 (Mouse); 25248 (Rat)

Alternate Names

CANN6; CANN6CB1R; Cannabinoid R1; cannabinoid receptor 1 (brain); CannabinoidR1; CB1; CB1A; CB1K5; CB1R; CB-R; CB-RCB1cannabinoid receptor 1; central cannabinoid receptor; CNR; CNR1; SKR6R