Human Cannabinoid R1/CB1/CNR1 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB3834A
Detection of Cannabinoid R1/CB1/CNR1 in NS0 Mouse Cell Line Transfected with Human Cannabinoid R1/CB1/CNR1 and eGFP by Flow Cytometry.
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Human Cannabinoid R1/CB1/CNR1 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Stains Cannabinoid R1/CB1/CNR1-transfected cells but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2A Clone # 368302
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HEK293 human embryonic kidney cell line transfected with human Cannabinoid R1/CB1/CNR1
Met1-Leu472
Accession # NP_057167
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Cannabinoid R1/CB1/CNR1 antibody in NS0 Mouse Cell Line Transfected with Human Cannabinoid R1/CB1/CNR1 and eGFP antibody by Flow Cytometry. View Larger

Detection of Cannabinoid R1/CB1/CNR1 in NS0 Mouse Cell Line Transfected with Human Cannabinoid R1/CB1/CNR1 and eGFP by Flow Cytometry. NS0 mouse myeloma cell line transfected with human Cannabinoid R1/CB1/CNR1 and eGFP was stained with either (A) Mouse Anti-Human Cannabinoid R1/CB1/CNR1 APC-conjugated Monoclonal Antibody (Catalog # FAB3834A) or (B) Mouse IgG2AAllophycocyanin Isotype Control (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Cannabinoid R1/CB1/CNR1

Cannabinoid R1 (CB1R) is a 60 kDa 7TM protein that belongs to the family of G protein-coupled receptors, class A. CB1R is expressed in the central nervous system and upper GI tract, in contrast to CB2R which is expressed by hematopoietic cells. CB1R mediates the behavioral and gut motility effects of cannabinoids. Human CB1R exists in three alternately spliced forms which are distinguished by N-terminal substitutions or deletions. Human CB1R shares 97% amino acid sequence identity with mouse and rat CB1R.

Long Name
Cannabinoid Receptor 1
Entrez Gene IDs
1268 (Human); 12801 (Mouse); 25248 (Rat)
Alternate Names
CANN6; CANN6CB1R; Cannabinoid R1; cannabinoid receptor 1 (brain); CannabinoidR1; CB1; CB1A; CB1K5; CB1R; CB-R; CB-RCB1cannabinoid receptor 1; central cannabinoid receptor; CNR; CNR1; SKR6R

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