Human Carbonic Anhydrase XIII/CA13 Antibody

Catalog #: AF2194 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2194
AF2194-SP

All Carbonic Anhydrase XIII/CA13 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Carbonic Anhydrase XIII/CA13 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Carbonic Anhydrase XIII/CA13 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross‑reactivity with recombinant human (rh) CA1, rhCA2, and rhCA3 is observed and 5% cross‑reactivity with rhCA4, rhCA8, rhCA9, rhCA10, rhCA12, and rhCA14 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Carbonic Anhydrase XIII/CA13
Ser2-His262
Accession # Q8N1Q1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Carbonic Anhydrase XIII (Catalog # 2194-CA)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Carbonic Anhydrase XIII (Catalog # 2194-CA), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbonic Anhydrase XIII/CA13

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6^th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO₂ removal and mosquito control in industry. CA13 is a cytosolic enzyme with a unique and widespread distribution pattern as compared to the other cytosolic CAs, indicating that it may play important physiological roles in several organs (2).

References

Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
Lehtonen, J. et al. (2003) J. Biol. Chem. 279: 2719.

Entrez Gene IDs

377677 (Human)

Alternate Names

CA13; carbonic anhydrase 13; Carbonic Anhydrase XIII; carbonic anhydrase XIIICarbonate dehydratase XIII; CAXIII; CA-XIII; EC 4.2.1.1; FLJ37995; MGC59868