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Human Cardiac Troponin I/TNNI3 Antibody

Catalog #: MAB6887
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB6887
MAB6887-SP
Detection of Human Cardiac Troponin I/TNNI3 by Western Blot.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Cardiac Troponin I/TNNI3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Cardiac Troponin I/TNNI3 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2B Clone # 679013
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human Cardiac Troponin I/TNNI3 purified from human heart
Accession # P19429
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Cardiac Troponin I antibody by Western Blot. View Larger

Detection of Human Cardiac Troponin I/TNNI3 by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Cardiac Troponin I/TNNI3 Monoclonal Antibody (Catalog # MAB6887) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Cardiac Troponin I/TNNI3 at approximately 24-29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Troponin I/TNNI3

Troponin I, also known as TNI, is a 24-29 kDa component of a protein complex on striated muscle thin filaments. Troponin I inhibits the calcium-dependent muscle contraction mediated by Troponins C and T. The expression of cardiac Troponin I (TNNI3) is restricted to cardiac muscle, while TNNI1 and TNNI2 (encoded by distinct genes) are expressed in skeletal muscle. Mutations of cardiac Troponin I are associated with heriditary cardiomyopathy. Human cardiac Troponin I shares 93% amino acid sequence identity with mouse and rat cardiac Troponin I.

Long Name
Troponin I3, Cardiac Type
Entrez Gene IDs
7137 (Human)
Alternate Names
Cardiac troponin I; CMD1FF; CMD2A; CMH7MGC116817; CTnI; familial hypertrophic cardiomyopathy 7; RCM1; TNNC1; TNNC1troponin I, cardiac; TNNI3,; troponin I type 3 (cardiac); troponin I, cardiac muscle; Troponin I/TNNI3

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Citations for Human Cardiac Troponin I/TNNI3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Wnt inhibition promotes vascular specification of embryonic cardiac progenitors
    Authors: DE Reichman, L Park, L Man, D Redmond, K Chao, RP Harvey, MM Taketo, Z Rosenwaks, D James
    Development, 2018-01-08;145(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Human embryonic stem cell derived mesenchymal progenitors express cardiac markers but do not form contractile cardiomyocytes.
    Authors: Raynaud C, Halabi N, Elliott D, Pasquier J, Elefanty A, Stanley E, Rafii A
    PLoS ONE, 2013-01-16;8(1):e54524.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  3. Quantification of Etoposide Hypersensitivity: A Sensitive, Functional Method for Assessing Pluripotent Stem Cell Quality
    Authors: Frank J. Secreto, Xing Li, Alyson J. Smith, Elizabeth S. Bruinsma, Ester Perales-Clemente, Saji Oommen et al.
    Stem Cells Translational Medicine

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