Human Cardiotrophin-1/CT-1 Antibody

Catalog #: MAB2601
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2601-SP
MAB2601-500
MAB2601-100

Cell Proliferation Induced by Cardiotrophin‑1/CT‑1 and Neutralization by Human Cardiotrophin‑1/CT‑1 Antibody.

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Cardiotrophin-1/CT-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Cardiotrophin‑1/CT‑1 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 89215

Purification

Protein A or G purified from ascites

Immunogen

E. coli-derived recombinant human Cardiotrophin‑1/CT‑1
Ser2-Ala201
Accession # Q16619

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Cardiotrophin‑1/CT‑1 (Catalog # 612-CD)

Neutralization

Measured by its ability to neutralize Cardiotrophin‑1/CT‑1-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 1-3 µg/mL in the presence of 20 ng/mL Recombinant Human Cardiotrophin‑1/CT‑1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by Cardiotrophin‑1/CT‑1 and Neutralization by Human Cardiotrophin‑1/CT‑1 Antibody. Recombinant Human Cardiotrophin-1/CT-1 (Catalog # 612-CD) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Cardiotrophin-1/CT-1 (20 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Cardiotrophin-1/CT-1 Mono- clonal Antibody (Catalog # MAB2601). The ND₅₀ is typically 1-3 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cardiotrophin-1/CT-1

Cardiotrophin-1 (CT-1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). It was originally isolated based on its ability to induce cardiac myocyte hypertrophy in vitro. CT-1 has since been shown to be a pleiotrophic cytokine with overlapping actions with other IL-6 family members on a variety of cell types. Human CT-1 encodes a 201 amino acid (aa) residue protein that lacks a hydrophobic signal peptide. The mechanism of CT-1 release from cells is currently not understood. Human and mouse CT-1 share 80% aa sequence identity and exhibit cross-species activity. CT-1 is highly expressed in heart, skeletal muscle, liver, lung and kidney. Lower levels of CT-1 expression is also seen in testis and brain. CT-1 initiates downstream signaling pathways through the heterodimerization of gp130 and the LIF receptor beta subunit. A third alpha receptor subunit has been implicated in the receptor complex.

References

Pennica, D. et al. (1996) Cytokine and Growth Factor Reviews 7:81.
Robledo, O. et al. (1997) J. Biol. Chem. 272:4855.

Entrez Gene IDs

1489 (Human); 13019 (Mouse)

Alternate Names

cardiophin 1; cardiotrophin 1; Cardiotrophin1; Cardiotrophin-1; CT-1CT1; CTF1

Product Datasheets

Product Datasheet

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Related Research Areas

Citation for Human Cardiotrophin-1/CT-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Cardiotrophin-1 in choroid plexus and the cerebrospinal fluid circulatory system.
Authors: Gard AL, Gavin E, Solodushko V, Pennica D
Neuroscience, 2004-01-01;127(1):43-52.
Species: Mouse, Rat
Sample Types: Tissue Homogenates
Applications: Western Blot