Human Cardiotrophin-1/CT-1 Antibody

Name: Human Cardiotrophin-1/CT-1 Antibody
Brand: R&D Systems
SKU: MAB260
Rating: 5 (1 reviews)

Catalog #: MAB260
1 Citation 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB260
MAB260-SP

All Cardiotrophin-1/CT-1 Antibodies

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Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Cardiotrophin-1/CT-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Cardiotrophin‑1/CT‑1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Cardiotrophin-1/CT-1 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 89228

Purification

Protein A or G purified from ascites

Immunogen

E. coli-derived recombinant human Cardiotrophin‑1/CT‑1
Ser2-Ala201
Accession # Q16619

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Cardiotrophin‑1/CT‑1 (Catalog # 612-CD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cardiotrophin-1/CT-1

Cardiotrophin-1 (CT-1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). It was originally isolated based on its ability to induce cardiac myocyte hypertrophy in vitro. CT-1 has since been shown to be a pleiotrophic cytokine with overlapping actions with other IL-6 family members on a variety of cell types. Human CT-1 encodes a 201 amino acid (aa) residue protein that lacks a hydrophobic signal peptide. The mechanism of CT-1 release from cells is currently not understood. Human and mouse CT-1 share 80% aa sequence identity and exhibit cross-species activity. CT-1 is highly expressed in heart, skeletal muscle, liver, lung and kidney. Lower levels of CT-1 expression is also seen in testis and brain. CT-1 initiates downstream signaling pathways through the heterodimerization of gp130 and the LIF receptor beta subunit. A third alpha receptor subunit has been implicated in the receptor complex.

References

Pennica, D. et al. (1996) Cytokine and Growth Factor Reviews 7:81.
Robledo, O. et al. (1997) J. Biol. Chem. 272:4855.

Entrez Gene IDs

1489 (Human); 13019 (Mouse)

Alternate Names

cardiophin 1; cardiotrophin 1; Cardiotrophin1; Cardiotrophin-1; CT-1CT1; CTF1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Cardiotrophin-1/CT-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1