Human Cardiotrophin-1/CT-1 Antibody

Catalog #: MAB2603 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2603-100
MAB2603-SP

Human Cardiotrophin-1/CT-1 ELISA Standard Curve.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Cardiotrophin-1/CT-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Cardiotrophin‑1/CT‑1 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 89230

Purification

Protein A or G purified from ascites

Immunogen

E. coli-derived recombinant human Cardiotrophin‑1/CT‑1
Ser2-Ala201
Accession # Q16619

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA captureantibody when paired with Mouse Anti-Human Cardiotrophin-1/CT-1 Monoclonal Antibody (Catalog # MAB2602).

This product is intended for assay development onvarious assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human Cardiotrophin-1/CT-1 ELISA Standard Curve. Recombinant human Cardiotrophin/CT-1 protein was serially diluted 2-fold and captured by Mouse Anti-Human Cardiotrophin/CT-1 Monoclonal Antibody (Catalog # MAB2603) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Cardiotrophin/CT-1 Monoclonal Antibody (Catalog # MAB2602) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cardiotrophin-1/CT-1

Cardiotrophin-1 (CT-1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). It was originally isolated based on its ability to induce cardiac myocyte hypertrophy in vitro. CT-1 has since been shown to be a pleiotrophic cytokine with overlapping actions with other IL-6 family members on a variety of cell types. Human CT-1 encodes a 201 amino acid (aa) residue protein that lacks a hydrophobic signal peptide. The mechanism of CT-1 release from cells is currently not understood. Human and mouse CT-1 share 80% aa sequence identity and exhibit cross-species activity. CT-1 is highly expressed in heart, skeletal muscle, liver, lung and kidney. Lower levels of CT-1 expression is also seen in testis and brain. CT-1 initiates downstream signaling pathways through the heterodimerization of gp130 and the LIF receptor beta subunit. A third alpha receptor subunit has been implicated in the receptor complex.

References