Human Cardiotrophin-1/CT-1 Antibody

Catalog #: AF612
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF612
AF612-SP

Cell Proliferation Induced by Cardiotrophin‑1/CT‑1 and Neutralization by Human Cardiotrophin‑1/CT‑1 Antibody.

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Citations (3)

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Human Cardiotrophin-1/CT-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Cardiotrophin‑1/CT‑1 in Western blots. In Western blots, approximately 50% cross-reactivity with recombinant mouse Cardiotrophin-1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Cardiotrophin-1/CT-1
Ser2-Ala201
Accession # Q16619

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Cardiotrophin‑1/CT‑1 (Catalog # 612-CD)

Neutralization

Measured by its ability to neutralize Cardiotrophin‑1/CT‑1-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 0.1‑0.3 µg/mL in the presence of 15 ng/mL Recombinant Human Cardiotrophin‑1/CT‑1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by Cardiotrophin‑1/CT‑1 and Neutralization by Human Cardiotrophin‑1/CT‑1 Antibody. Recombinant Human Cardiotrophin-1/CT-1 (Catalog # 612-CD) stimulates proliferation in the TF-1 human erythro-leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Cardiotrophin-1/CT-1 (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Cardiotrophin-1/CT-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF612). The ND₅₀ is typically 0.1-0.3 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cardiotrophin-1/CT-1

Cardiotrophin-1 (CT-1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). It was originally isolated based on its ability to induce cardiac myocyte hypertrophy in vitro. CT-1 has since been shown to be a pleiotrophic cytokine with overlapping actions with other IL-6 family members on a variety of cell types. Human CT-1 encodes a 201 amino acid (aa) residue protein that lacks a hydrophobic signal peptide. The mechanism of CT-1 release from cells is currently not understood. Human and mouse CT-1 share 80% aa sequence identity and exhibit cross-species activity. CT-1 is highly expressed in heart, skeletal muscle, liver, lung and kidney. Lower levels of CT-1 expression is also seen in testis and brain. CT-1 initiates downstream signaling pathways through the heterodimerization of gp130 and the LIF receptor beta subunit. A third alpha receptor subunit has been implicated in the receptor complex.

References

Pennica, D. et al. (1996) Cytokine and Growth Factor Reviews 7:81.
Robledo, O. et al. (1997) J. Biol. Chem. 272:4855.

Entrez Gene IDs

1489 (Human); 13019 (Mouse)

Alternate Names

cardiophin 1; cardiotrophin 1; Cardiotrophin1; Cardiotrophin-1; CT-1CT1; CTF1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Cardiotrophin-1/CT-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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HIF-1-mediated up-regulation of cardiotrophin-1 is involved in the survival response of cardiomyocytes to hypoxia.
Authors: Robador PA, San Jose G, Rodriguez C, Guadall A, Moreno MU, Beaumont J, Fortuno A, Diez J, Martinez-Gonzalez J, Zalba G
Cardiovasc. Res., 2011-07-19;92(2):247-55.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Cardiotrophin-1 induces interleukin-6 synthesis in human monocytes.
Authors: Fritzenwanger M, Meusel K, Foerster M, Kuethe F, Krack A, Figulla HR
Cytokine, 2007-07-16;38(3):137-44.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Alterations in Janus kinase (JAK)-signal transducers and activators of transcription (STAT) signaling in patients with end-stage dilated cardiomyopathy.
Authors: Podewski EK, Hilfiker-Kleiner D, Hilfiker A, Morawietz H, Lichtenberg A, Wollert KC, Drexler H
Circulation, 2003-02-18;107(6):798-802.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot