Human Caspase-10 Antibody

Catalog #: MAB834
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB834
MAB834-SP

Detection of Human Caspase‑10 by Western Blot.

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All Caspase-10 Antibodies

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Caspase-10 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Caspase-10 in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 63131

Purification

Protein A or G purified from ascites

Immunogen

E. coli-derived recombinant human Caspase-10/a/B (Mch 4)
Val220-Ile479
Accession # AAC50644

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Caspase‑10 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with Human FAS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF126) for for the indicated times. PVDF membrane was probed with 1 µg/mL of Human Caspase-10 Monoclonal Antibody (Catalog # MAB834), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Caspase-10 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Caspase-10

Caspase-10 (Cysteine-aspartic acid protease 10, FLICE2 and Mch4) is a 28-29 kDa member of the peptidase C14A family of enzymes. It is widely expressed, being found in blood and epithelial cells. Caspase-10 appears to be an initiator caspase that acts on Pro-Caspases-3, -4, -6, -7, and -9. Human Pro-Caspase-10a is a 59 kDa, 521 amino acid (aa) protein. It contains two N-terminal death domains. Cleavage generates p43 plus the small mature p12 subunit. Cleavage of p43 generates a prodomain p25 and large mature p17 subunit. P17 and p12 noncovalently associate to form a 29 kDa mature heterodimer. There are at least four isoform variants. There is no apparent mouse Casp-10 gene.

Entrez Gene IDs

843 (Human)

Alternate Names

Apoptotic protease Mch-4; CASP10; CASP-10; caspase 10, apoptosis-related cysteine peptidase; caspase 10, apoptosis-related cysteine protease; Caspase10; Caspase-10; EC 3.4.22.63; FADD-like ICE2; FAS-associated death domain protein interleukin-1B-converting enzyme 2; FLICE2; ICE-like apoptotic protease 4; interleukin-1B-converting enzyme 2; Mch4; MCH4ALPS2

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human Caspase-10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Identification of caspase-10 in human neutrophils and its role in spontaneous apoptosis.
Authors: Goepel F, Weinmann P, Schymeinsky J, Walzog B
J. Leukoc. Biol., 2004-02-03;75(5):836-43.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Upregulation of the Fas receptor death-inducing signaling complex after traumatic brain injury in mice and humans.
Authors: Qiu J, Whalen MJ, Lowenstein P, Fiskum G, Fahy B, Darwish R, Aarabi B, Yuan J, Moskowitz MA
J. Neurosci., 2002-05-01;22(9):3504-11.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot