Human Caspr2 Antibody

Catalog #: MAB5145 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5145-100
MAB5145-SP

Detection of Caspr2 in HEK293 Human Cell Line Transfected with Human Caspr2 and eGFP by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Caspr2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Caspr2 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 840727

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Caspr2
Pro29-Ala1262
Accession # Q9UHC6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Caspr2 in HEK293 Human Cell Line Transfected with Human Caspr2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human Caspr2 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Caspr2 Monoclonal Antibody (Catalog # MAB5145) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Caspr2

Caspr2 (CNTNAP2, Contactin-associated protein-like 2 and Cell recognition molecule Caspr2) is a 160-170 kDa glycoprotein, a member of the neurexin family of proteins. It is predominantly expressed in nervous system and likely play a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Seems to demarcate the juxtaparanodal region of the axo-glial junction. Caspr2 and closely related molecule Caspr/Paranodin demarcate distinct subdomains in myelinated axons. While contactin-associated protein (Caspr) is present at the paranodal junctions, Caspr2 is precisely colocalized with Shaker-like K+ channels in the juxtaparanodal region. Over aa 29-1262, human CASPR2 shares 94% aa sequence identity with mouse Caspr2.

Long Name

Contactin Associated Protein-like 2

Entrez Gene IDs

26047 (Human); 66797 (Mouse)

Alternate Names

AUTS15; Caspr2; CASPR2DKFZp781D1846; CDFE; Cell recognition molecule Caspr2; CNTNAP2; contactin associated protein-like 2; contactin-associated protein 2; contactin-associated protein-like 2; homolog of Drosophila neurexin IV; KIAA0868; NRXN4; NRXN4Caspr2; PTHSL1