Human CCL16/HCC-4 Antibody Summary
Gln24-Gln120
Accession # O15467
Applications
Human CCL16/HCC-4 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Chemotaxis Induced by CCL16/HCC‑4 and Neutral-ization by Human CCL16/ HCC‑4 Antibody. Recombinant Human CCL16/ HCC-4 (Catalog # 802-HC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL16/ HCC-4 (0.75 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL16/ HCC-4 Monoclonal Antibody (Catalog # MAB328). The ND50 is typically 5-20 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL16/HCC-4
Human HCC-4, also named NCC-4, liver-expressed chemokine (LEC), and lymphocyte and monocyte chemoattractant (LMC), is a novel CC chemokine identified through bioinformatics. HCC-4 cDNA encodes a 120 amino acid (aa) residue precursor protein with a 23 aa residue predicted signal peptide that is cleaved to generate a 97 aa residue mature protein. HCC-4 is distantly related to other CC chemokines, exhibiting less than 30% aa sequence identity. Among these CC chemokines, HCC-4 has the most similarity to HCC-1. Two potential polyadenylation signals are present on the human HCC-4 gene, and as a result, two transcripts containing approximately 1,500 base pairs and 500 base pairs have been detected. HCC-4 is expressed weakly by some lymphocytes, including NK cells, gamma δ T cells, and some T cell clones. The expression of HCC-4 in monocytes is highly upregulated in the presence of IL-10. The HCC-4 gene has been mapped to chromosome 17q where multiple CC chemokines are clustered.
Recombinant HCC-4 has been shown to chemoattract human monocytes and THP-1 cells but not resting lymphocytes or neutrophils. HCC-4 has also been found to suppress proliferation of myeloid progenitor cells. The HCC-4 induced calcium flux in THP-1 cells can be desensitized by prior exposure to RANTES, suggesting that HCC-4 and RANTES share the same receptor in THP-1 cells.
- Shoudai, K. et al. (1998) Biochim. Biophys. Acta 1396:273.
- Hedrick, J. et al. (1998) Blood 91:4242.
- Youn, B-S. et al. (1998) BBRC 247:217.
Product Datasheets
Citations for Human CCL16/HCC-4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Structure and Dynamics of Human Chemokine CCL16-Implications for Biological Activity
Authors: OH Weiergräbe, D Petrovi?, A Kislat, M Pattky, J Fabig, R Batra-Saff, J Schulte Am, K Hänel, C Huhn, B Strodel, B Homey, D Willbold
Biomolecules, 2022-10-28;12(11):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
CCL16/LEC powerfully triggers effector and antigen-presenting functions of macrophages and enhances T cell cytotoxicity.
Authors: Cappello P, Caorsi C, Bosticardo M
J. Leukoc. Biol., 2003-10-02;75(1):135-42.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
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