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Human CCL22/MDC PE-conjugated Antibody

Catalog #: IC3361P
6 Citations Datasheet / COA / SDS
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IC3361P
Detection of CCL22/MDC in Human Dendritic Cells by Flow Cytometry.
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Citations (6)
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Human CCL22/MDC PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL22/MDC in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant cotton rat CCL3, 4, 5, recombinant human CCL1, 2, 3, 4, 5, 7, 8,  9/10/MIP-1 gamma, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 23, 24, 25, 26, 27, 28, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 8, 9/10/MIP-1 gamma, 11, 12, 17, 19, 21, 22, 24, 25, 27, 28, or recombinant rat CCL20.
Source
Monoclonal Mouse IgG2B Clone # 57203
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human CCL22/MDC
Gly25-Gln93
Accession # O00626.1
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of CCL22/MDC antibody in Human Dendritic Cells antibody by Flow Cytometry. View Larger

Detection of CCL22/MDC in Human Dendritic Cells by Flow Cytometry. Human dendritic cells treated with LPS, Recombinant Human TNF-a (Catalog # 210-TA), and Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB) were stained with Mouse Anti-Human CCL22/MDC PE-conjugated Monoclonal Antibody (Catalog # IC3361P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCL22/MDC

CCL22, also named stimulated T cell chemotactic protein (STCP-1), is a CC chemokine initially isolated from clones of monocyte-derived macrophages. Human CCL22 cDNA encodes a precursor protein of 93 amino acid residues with a 24 amino acid residue predicted signal peptide that is cleaved to yield a 69 amino acid residue mature 8 kDa protein. At the amino acid sequence level, CCL22 shows less than 35% identity to other CC chemokine family members. Human CCL22 is expressed in dendritic cells, macrophages and activated monocytes. In addition, CCL22 expression is also detected in the tissues of thymus, lymph node and appendix. The gene for human CCL22 has been mapped to chromosome 16 rather than chromosome 17 where the genes for many human CC chemokines are clustered. Recombinant or chemically synthesized mature CCL22 has been shown to induce chemotaxis or Ca2+ mobilization in dendritic cells, IL-2 activated NK cells, and activated T lymphocytes. A CD8+ T lymphocyte-derived secreted soluble activity that suppresses infection by primary non-syncytium-inducing and syncytium-inducing HIV-1 isolates and the T-cell line-adapted isolate HIV-1IIIB, has been identified as CCL22. Based on amino-terminal sequence analysis, the major CD8+ T lymphocyte-derived CCL22 protein yielded an amino-terminal sequence of YGANM, which is two amino acid residues shorter than the predicted mature CCL22. The difference in potency between the two mature CCL22 isoforms has not been determined.

References
  1. Godiska, R. et al. (1997) J. Exp. Med. 185:1595.
  2. Chang, M-S. et al. (1997) J. Biol. Chem. 272:25229.
  3. Pal, R. et al. (1997) Science 278:5338.
Entrez Gene IDs
6367 (Human); 20299 (Mouse)
Alternate Names
A-152E5.1; ABCD-1; CC chemokine STCP-1; C-C motif chemokine 22; CCL22; chemokine (C-C motif) ligand 22; DC/B-CK; Macrophage-derived chemokine; MDC; MDCStimulated T-cell chemotactic protein 1; MGC34554; SCYA22MDC(1-69); small inducible cytokine A22; small inducible cytokine subfamily A (Cys-Cys), member 22; Small-inducible cytokine A22; STCP-1; stimulated T cell chemotactic protein 1

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Citations for Human CCL22/MDC PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. A CCR4 antagonist reverses the tumor-promoting microenvironment of renal cancer.
    Authors: Berlato C, Khan MN, Schioppa T et al.
    J. Clin. Invest.
  2. Human Decidual CD1a+ Dendritic Cells Undergo Functional Maturation Program Mediated by Gp96
    Authors: T Gulic, G Laskarin, L Glavan, T Grubi? Kez, H Haller, D Rukavina
    International Journal of Molecular Sciences, 2023-01-23;24(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. High-content cytometry and transcriptomic biomarker profiling of human B-cell activation.
    Authors: Hennig C, Ilginus C, Boztug K, Skokowa J, Marodi L, Szaflarska A, Sass M, Pignata C, Kilic S, Caragol I, Baumann U, Klein C, Welte K, Hansen G
    J Allergy Clin Immunol, 2013-09-05;133(1):172-80.e1-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Chip Cytometry
  4. Identification of human NK17/NK1 cells.
    Authors: Pandya AD, Al-Jaderi Z, Hoglund RA, Holmoy T, Harbo HF, Norgauer J, Maghazachi AA
    PLoS ONE, 2011-10-21;6(10):e26780.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010-02-08;120(3):907-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. CCL17 and CCL22 chemokines within tumor microenvironment are related to accumulation of Foxp3+ regulatory T cells in gastric cancer.
    Authors: Mizukami Y, Kono K, Kawaguchi Y, Akaike H, Kamimura K, Sugai H, Fujii H
    Int. J. Cancer, 2008-05-15;122(10):2286-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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