Human CCL28 Antibody

Catalog #: MAB7171
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7171
MAB7171-SP

All CCL28 Antibodies

Product Details

Citations (3)

Supplemental Products

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCL28 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CCL28 in ELISAs and Western blots. In sandwich immunoassays, no cross-reactivity or interference with recombinant human CCL27 or recombinant mouse CCL28 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 62714

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human CCL28
Ile23-Tyr127
Accession # Q9NRJ3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human CCL28 (Catalog # 717-VC)

Human CCL28 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human CCL28 Biotinylated Antibody (Catalog # BAF717)

Standard: Recombinant Human CCL28 Protein (Catalog # 717-VC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL28

Human CCL28 (CC chemokine ligand 28) is a member of the CC chemokine superfamily. Human CCL28 cDNA encodes a 127 amino acid (aa) residue precursor protein with a putative 22 aa residue signal peptide that is cleaved to produce the 105 aa residue mature protein. Human and mouse CCL28 share 83% aa identity in their mature regions. Among CC chemokines, CCL28 shares the most homology with CCL27/CTACK. Human and mouse CCL28 RNA is most highly expressed by epithelial cells in normal and pathologic colon. Human CCL28 RNA is also present in normal and asthmatic lung tissues. The receptor for CCL28 is the CCR10 (formerly GPR2 orphan receptor) which is also the receptor for CCL27/CTACK.

References

Wang, W. et al. (2000) J. Biol. Chem. 275:22313.

Entrez Gene IDs

56477 (Human); 56838 (Mouse)

Alternate Names

CCL28; chemokine (C-C motif) ligand 28; MEC; member 28; MGC71902; Protein CCK1; VIC

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human CCL28 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Th22 cells are efficiently recruited in the gut by CCL28 as an alternative to CCL20 but do not compensate for the loss of Th17 cells in treated HIV-1-infected individuals
Authors: M Nayrac, M Requena, C Loiseau, M Cazabat, B Suc, N Carrere, K Barange, L Alric, G Martin-Blo, J Izopet, P Delobel
Mucosal Immunol, 2020-04-28;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Cancer cell chemokines direct chemotaxis of activated stellate cells in pancreatic ductal adenocarcinoma
Authors: Ishan Roy, Kathleen A. Boyle, Emily P. Vonderhaar, Noah P. Zimmerman, Egal Gorse, A. Craig Mackinnon et al.
Laboratory Investigation
The Ovarian Cancer Chemokine Landscape Is Conducive to Homing of Vaccine-Primed and CD3/CD28-Costimulated T Cells Prepared for Adoptive Therapy.
Authors: Zsiros E, Duttagupta P, Dangaj D, Li H, Frank R, Garrabrant T, Hagemann I, Levine B, June C, Zhang L, Wang E, Marincola F, Bedognetti D, Powell D, Tanyi J, Feldman M, Kandalaft L, Coukos G
Clin Cancer Res, 2015-02-23;21(12):2840-50.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: IHC, Neutralization