Human CD11b/Integrin alpha M Antibody Summary
Phe17-Asn1105
Accession # NP_001139280
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CD11b/Integrin alpha M in Human Colon Cancer via seqIF™ staining on COMET™ CD11b/Integrin alpha M Antibody was detected in immersion fixed paraffin-embedded sections of human colon cancer using Mouse Anti-Human CD11b/Integrin alpha M Monoclonal Antibody (Catalog # MAB16992) at 15ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.
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Detection of CD11b/Integrin alpha M in Human Spleen via Multiplex Immunofluorescence staining on COMET™ CD11b/Integrin alpha M was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human CD11b/Integrin alpha M Monoclonal Antibody (MAB16992) at 15 μg/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.
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Detection of CD11b/Integrin alpha M in Human Tonsil via seqIF™ staining on COMET™ CD11b/Integrin alpha M Antibody was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD11b/Integrin alpha M Monoclonal Antibody (Catalog # MAB16992) at 15ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.
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Detection of CD11b/Integrin alpha M in Human Spleen. CD11b/Integrin alpha M was detected in formalin fixed paraffin embedded sections of human spleen using Mouse Anti-Human CD11b/Integrin alpha M Monoclonal Antibody (Catalog # MAB16992) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with Hematoxylin (blue). Specific staining was localized to cell surface and cytoplasm of lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD11b/Integrin alpha M
The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha and a beta subunit. The Integrin alpha M subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin alpha M/ beta 2, also known as MAC-1 and complement receptor type 3 (CR3). Integrin alpha M/ beta 2 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alpha M/ beta 2 can bind several ligands (including ICAM-1 fibrinogen and the C3 complement fragment C3bi) to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles.
Product Datasheets
Citations for Human CD11b/Integrin alpha M Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
9
Citations: Showing 1 - 9
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An integrated gene-to-outcome multimodal database for metabolic dysfunction-associated steatotic liver disease
Authors: Kendall, TJ;Jimenez-Ramos, M;Turner, F;Ramachandran, P;Minnier, J;McColgan, MD;Alam, M;Ellis, H;Dunbar, DR;Kohnen, G;Konanahalli, P;Oien, KA;Bandiera, L;Menolascina, F;Juncker-Jensen, A;Alexander, D;Mayor, C;Guha, IN;Fallowfield, JA;
Nature medicine
Species: Human
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
The Effect of Heterozygous Mutation of Adenylate Kinase 2 Gene on Neutrophil Differentiation
Authors: T Horiguchi, A Tanimura, K Miyoshi, H Hagita, H Minami, T Noma
International Journal of Molecular Sciences, 2022-12-17;23(24):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Synergism between obesity and HFpEF on neutrophils phenotype and its regulation by adipose tissue-molecules and SGLT2i dapagliflozin
Authors: C Almengló, X Fu, MT Flores-Ari, ÁL Fernández, JE Viñuela, JM Martínez-C, D Durán, M Rodríguez-, JR González-J, S Eiras
Journal of Cellular and Molecular Medicine, 2022-07-11;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
A Novel Triple-Cell Two-Dimensional Model to Study Immune-Vascular Interplay in Atherosclerosis
Authors: Jonathan Noonan, Gianluca Grassia, Neil MacRitchie, Paul Garside, Tomasz J. Guzik, Angela C. Bradshaw et al.
Frontiers in Immunology
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Differential expression of adenine nucleotide converting enzymes in mitochondrial intermembrane space: a potential role of adenylate kinase isozyme 2 in neutrophil differentiation.
Authors: Tanimura, Ayako, Horiguchi, Taigo, Miyoshi, Keiko, Hagita, Hiroko, Noma, Takafumi
PLoS ONE, 2014-02-25;9(2):e89916.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
1,25-Dihydroxyvitamin D3 promotes a sustained LPS-induced NF-kappaB-dependent expression of CD55 in human monocytic THP-1 cells.
Authors: Izban M, Nowicki B, Nowicki S
PLoS ONE, 2012-11-12;7(11):e49318.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Dose-dependent changes in neuroinflammatory and arachidonic acid cascade markers with synaptic marker loss in rat lipopolysaccharide infusion model of neuroinflammation.
BMC Neurosci, 2012-05-23;13(0):50.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Lyn-coupled LacCer-enriched lipid rafts are required for CD11b/CD18-mediated neutrophil phagocytosis of nonopsonized microorganisms.
Authors: Nakayama H, Yoshizaki F, Prinetti A, Sonnino S, Mauri L, Takamori K, Ogawa H, Iwabuchi K
J. Leukoc. Biol., 2007-11-30;83(3):728-41.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Protein-tyrosine kinase Syk is required for pathogen engulfment in complement-mediated phagocytosis.
Authors: Shi Y, Tohyama Y, Kadono T, He J, Shahjahan Miah SM, Hazama R, Tanaka C, Tohyama K, Yamamura H
Blood, 2006-01-31;107(11):4554-62.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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