Human CD155/PVR Antibody

Catalog #: MAB2530
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2530
MAB2530-SP

Detection of Human CD155/PVR by Western Blot.

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All CD155/PVR Antibodies

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD155/PVR Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD155/PVR in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 300903

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD155/PVR
Gly27-Asn343
Accession # ABM85069

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human CD155/PVR by Western Blot. Western blot shows lysates of human heart tissue, HT1080 human fibrosarcoma cell line, and HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human CD155/PVR Monoclonal Antibody (Catalog # MAB2530) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD155/PVR at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD155/PVR

CD155 [also known as PVR (poliovirus receptor) and Necl-5 (nectin-like molecule-5)] is a 70 kDa type I transmembrane (TM) glycoprotein that is a member of the nectin-like (Necl) family of nectin-related molecules (1). Like nectins, Necl molecules are Ig superfamily members that contain three Ig-like extracellular domains, a TM segment, and a cytoplasmic tail. Unlike nectins, Necl molecules cannot interact with cytoplasmic afadin (1). While Nectins serve as cell adhesion molecules, the actual functions of most Necls are yet-to-be determined. CD155/PVR was originally isolated based on its ability to mediate polio virus attachment to host cells (2, 3). The full-length (or CD155 alpha isoform) is synthesized as a 417 amino acid (aa) precursor that contains a 20 aa signal sequence, a 323 aa extracellular region, a 24 aa TM segment and a 50 aa cytoplasmic tail. The extracellular region contains one N-terminal V-type and two C2-type Ig-like domains (2, 3). The V-type domain mediates polio virus binding (4). Three other isoforms exist, all of which retain the Ig-like domains. CD155δ is transmembrane with a shortened cytoplasmic tail of 25 aa. CD155 beta (352 aa) and CD155 gamma (344 aa) are 60‑65 kDa soluble forms that show removal of the TM segment and surrounding amino acids (2, 5). The soluble forms will bind the polio virus (due to the presence of the V-type Ig domain) but afford no protection against polio infection because of low circulating levels (5). CD155 has been demonstrated to bind vitronectin, nectin-3, and DNAM-1 (6‑8). DNAM-1 binding promotes monocyte migration and NK cell killing. CD155 is expressed in all normal tissues and is highly expressed in tumor cells of epithelial and neuronal origin.

References

Takai, Y. et al. (2003) Cancer Sci. 94:655.
Mendelsohn, C.L. et al. (1989) Cell 56:855.
Koike, H. et al. (1990) EMBO J. 9:3217.
Koike, S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:4104.
Baury, B. et al. (2003) Biochem. Biophys. Res. Commun. 309:175.
Mueller, S. and E. Wimmer (2003) J. Biol. Chem. 278:31251.
Reymond, N. et al. (2004) J. Exp. Med. 199:1331.
Lange, R. et al. (2001) Virology 285:218.

Long Name

Poliovirus Receptor

Entrez Gene IDs

5817 (Human); 52118 (Mouse); 25066 (Rat); 102136444 (Cynomolgus Monkey); 712851 (Rhesus Macaque)

Alternate Names

CD155 antigen; CD155; HVED; NECL5; Necl-5; nectin-like 5; Nectin-like protein 5; poliovirus receptor; PVR; PVS; PVSFLJ25946; Tage4

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human CD155/PVR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Targetable mechanisms driving immunoevasion of persistent senescent cells link chemotherapy-resistant cancer to aging
Authors: DP Muñoz, SM Yannone, A Daemen, Y Sun, F Vakar-Lope, M Kawahara, AM Freund, F Rodier, JD Wu, PY Desprez, DH Raulet, PS Nelson, LJ van 't Vee, J Campisi, JP Coppé
JCI Insight, 2019-06-11;5(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
The Cell Adhesion Molecule Necl-4/CADM4 Serves as a Novel Regulator for Contact Inhibition of Cell Movement and Proliferation.
Authors: Yamana, Shota, Tokiyama, Amina, Mizutani, Kiyohito, Hirata, Ken-ichi, Takai, Yoshimi, Rikitake, Yoshiyuk
PLoS ONE, 2015-04-20;10(4):e0124259.
Species: Human, Mouse
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC, Western Blot