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Human CD157 Antibody

Catalog #: AF4736
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4736
AF4736-SP
Detection of Human CD157 by Western Blot.
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Product Details
Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD157 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD157 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD157
Gly29-Lys292
Accession # Q10588
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human CD157 antibody by Western Blot. View Larger

Detection of Human CD157 by Western Blot. Western blot shows lysates of human kidney (cortex) tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human CD157 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4736) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD157 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD157

CD157, also known as bone marrow stromal cell antigen 1 (BST-1), is a glycosyl phosphatidylinositol anchored membrane protein that belongs to the CD38 family (1). CD157 was discovered in a bone marrow stromal cell line where it facilitates pre-B-cell growth (2, 3). Along with CD38, CD157 is a bifunctional ectoenzyme that exhibits both ADP-ribosyl cyclase and cyclic ADP ribose hydrolase activities (2). It may play a role in rheumatoid arthritis (RA) due to its enhanced expression in RA‑derived bone marrow stromal cell lines (3). CD157 has been predicted to function as a cell surface receptor and an immunoregulatory molecule (4).

References
  1. Hussain, A. M. M. et al. (1998) Protein Express. Purif. 12:133.
  2. Sato, A. et al. (1999) Biochem. J. 337:491.
  3. Kaisho, T. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5325.
  4. Ortolan, E. et al. (2002) Cell Biochem. Funct. 20:309.
Long Name
Cluster of Differentiation 157
Entrez Gene IDs
683 (Human); 12182 (Mouse); 81506 (Rat)
Alternate Names
ADP-ribosyl cyclase 2; Bone marrow stromal antigen 1; bone marrow stromal cell antigen 1; BP-3; BST1; BST-1; cADPr hydrolase 2; CD157 antigen; CD157; Cyclic ADP-ribose hydrolase 2; EC 3.2.2.5; NAD(+) nucleosidase

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Citations for Human CD157 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. NAD+ augmentation restores mitophagy and limits accelerated aging in Werner syndrome
    Authors: EF Fang, Y Hou, S Lautrup, MB Jensen, B Yang, T SenGupta, D Caponio, R Khezri, TG Demarest, Y Aman, D Figueroa, M Morevati, HJ Lee, H Kato, H Kassahun, JH Lee, D Filippelli, MN Okur, A Mangerich, DL Croteau, Y Maezawa, CA Lyssiotis, J Tao, K Yokote, TE Rusten, MP Mattson, H Jasper, H Nilsen, VA Bohr
    Nat Commun, 2019-11-21;10(1):5284.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Human canonical CD157/Bst1 is an alternatively spliced isoform masking a previously unidentified primate-specific exon included in a novel transcript
    Authors: E Ferrero, N Lo Buono, S Morone, R Parrotta, C Mancini, A Brusco, A Giacomino, S Augeri, A Rosal-Vela, S García-Rod, M Zubiaur, J Sancho, A Fiorio Pla, A Funaro
    Sci Rep, 2017-11-21;7(1):15923.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Binding of CD157 protein to fibronectin regulates cell adhesion and spreading.
    Authors: Morone S, Augeri S, Cuccioloni M, Mozzicafreddo M, Angeletti M, Lo Buono N, Giacomino A, Ortolan E, Funaro A
    J Biol Chem, 2014-04-21;289(22):15588-601.
    Species: Human
    Sample Types: Protein
    Applications: ELISA Development (Capture)

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