Human CD161 PE-conjugated Antibody

Name: Human CD161 PE-conjugated Antibody
Brand: R&D Systems
SKU: FAB7448P

Catalog #: FAB7448P Datasheet / COA / SDS

Discontinued Product

FAB7448P has been discontinued.

View all CD161 products.

Detection of CD161 in Human PBMCs by Flow Cytometry.

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All CD161 Antibodies

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Human CD161 PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD161 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 702228

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

BaF3 mouse pro-B cell line transfected with human CD161
Accession # Q12918

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD161 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD161 PE-conjugated Monoclonal Antibody (Catalog # FAB7448P) and Mouse Anti-Human CD4 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3791F). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD161

CD161, also known as NKR-P1A and KLRB1, is a 40 kDa type II transmembrane glycoprotein that contains one C-type lectin domain in its extracellular region. CD161 is expressed as a disulfide-linked dimer on the surface of Th17 cells and NK cells as well as on subsets of CD1-restricted T cells, intestinal NT cells, peripheral memory T cells, monocytes, and dendritic cells. It binds to OCIL/CLEC2d, leading to an inhibition of NK cell-mediated cytotoxicity and IFN-g secretion. Alternatively, CD161 can enhance TCR activation to CD1d ligation. CD161⁺ cell populations are depleted in ulcerative colitis, Grave’s disease, and AIDS, although CD161⁺ T cells are activated during asthmatic attacks. Additional related proteins are expressed in mouse but not human: the inhibitory NKR‑P1B and D, and the stimulatory NKR‑P1A, C, and F. Human CD161 shares 47% aa sequence identity with mouse and rat NKR-P1A.

Entrez Gene IDs

3820 (Human); 25192 (Rat)

Alternate Names

CD161 antigen; CD161; CLEC5B; CLEC5BC-type lectin domain family 5 member B; HNKR-P1a; killer cell lectin-like receptor subfamily B member 1; killer cell lectin-like receptor subfamily B, member 1; KLRB1; Ly59; Natural killer cell surface protein P1A; NKR; NKRP1; NKR-P1; NKRP1A; NKR-P1AMGC138614; NKRP1ANKR