Human CD20 mFluor™ Violet 450-Conjugated Antibody

Catalog #: FAB4225MFV450 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB4225MFV450-100

Detection of CD20 in PBMC lymphocytes by Flow Cytometry

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Human CD20 mFluor™ Violet 450-Conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD20. Stains human CD20 transfectants but not irrelevant transfectants.

Source

Monoclonal Mouse IgG₁ Clone # 396444

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human CD20
Met1-Pro297
Accession # P11836

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

mFluor Violet 450 SE (Excitation= 406 nm, Emission= 445 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.1-1 µg/10⁶ cells

PBMC lymphocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

CD20 Antibody (396444) [mFluor Violet 450 SE]

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Detection of CD20 in PBMC lymphocytes by Flow Cytometry PBMC lymphocytes were stained with Mouse Anti-Human CD19 PE‑conjugated Monoclonal Antibody (Catalog # FAB4867P) and either (A) Mouse Anti-Human CD20 mFluor™ Violet 450-Conjugated Monoclonal Antibody (Catalog # FAB4225MFV450) or (B) unstained. View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD20

CD20 is a 297 amino acid (aa) non-glycosylated type III membrane protein that is the founding member of the MS4A tetraspanin protein family. It is expressed on pre-B, naïve and mature B lymphocytes and B-cell lymphomas. CD20 is associated with lipid rafts upon crosslinking, and appears to play a role in regulation of ion influx. The one extracellular segment of 48 aa shows 65% aa sequence identity between human and mouse CD20.

Long Name

Cluster of Differentiation 20

Entrez Gene IDs

931 (Human); 12482 (Mouse); 25316 (Rat); 485430 (Canine); 102118491 (Cynomolgus Monkey)

Alternate Names

B1; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; B-lymphocyte surface antigen B1; Bp35; Bp35MGC3969; CD20 antigen; CD20 receptor; CD20; CD20S7; CVID5; LEU-16; Leukocyte surface antigen Leu-16; Ly-44; Membrane-spanning 4-domains subfamily A member 1; membrane-spanning 4-domains, subfamily A, member 1; MS4A1; MS4A2; S7