Human CD30/TNFRSF8 F(ab')2 (Research Grade Brentuximab Biosimilar) Antibody

Catalog #: MAB11129-FAB2 Datasheet / COA / SDS

Recombinant Monoclonal Antibody For Research Use Only

Catalog #	Availability	Size / Price	Qty
MAB11129-FAB2-100
MAB11129-FAB2-SP

Detection of CD30/TNFRSF8 in Jurkat human acute T cell leukemia cell line by Flow Cytometry.

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Human CD30/TNFRSF8 F(ab')2 (Research Grade Brentuximab Biosimilar) Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD30/TNFRSF8 in direct ELISAs. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Brentuximab. This product is for research use only.

Source

Recombinant Monoclonal Human IgG₁ Clone # Hu8F2

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human CD30/TNFRSF8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/mL

Jurkat human acute T cell leukemia cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD30/TNFRSF8 in Jurkat human acute T cell leukemia cell line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Human Anti-Human CD30/TNFRSF8 (Brentuximab Biosimilar) F(ab')2 Monoclonal Antibody (Catalog # MAB11129-FAB2, filled histogram) or no antibody (open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (F0135). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD30/TNFRSF8

Brentuximab is a biosimilar directed against the extracellular domain of CD30.

Entrez Gene IDs

943 (Human); 21941 (Mouse); 25069 (Rat)

Alternate Names

CD30 antigen; CD30; CD30KI-1; CD30L receptor; cytokine receptor CD30; D1S166EKi-1; Ki-1 antigen; Lymphocyte activation antigen CD30; TNFRSF8; tumor necrosis factor receptor superfamily member 8; tumor necrosis factor receptor superfamily, member 8