Human CD300g/Nepmucin Antibody

Catalog #: AF5328 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5328
AF5328-SP

Detection of Human CD300g/Nepmucin by Western Blot.

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Human CD300g/Nepmucin Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD300g/Nepmucin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse CD300g/Nepmucin is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD300g/Nepmucin
Leu19-Leu249
Accession # Q6UXG3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human CD300g/Nepmucin by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CD300g/Nepmucin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5328) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for CD300g/Nepmucin at approximately 75-95 kDa and 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD300g/Nepmucin

Nepmucin (A mucin not expressed in Peyer's Patches; also CD300g, CLM9, and TREM4) is a 75-95 kDa, O-glycosylated member of the CD300 family of molecules. It is expressed on capillary endothelium, and appears to serve multiple functions. Via a mucin domain, it binds lymphocyte L-selectin, contributing to cell migration. It also binds immunoglobulins A and M, perhaps mediating transendothelial transport. Mature human nepmucin is a 314 amino acid (aa ) type I transmembrane glycoprotein. It contains one V-type Ig-like domain (aa 19-121), a mucin stalk (aa 137-237), and a cytoplasmic region (aa 269-332). There are at least two isoform variants of nepmucin. Each shows a deletion of aa 128-212, accompanied by either a 23 aa, or 12 aa substitution for aa 296-332. Over aa 19-249, human nepmucin shares 51% aa identity with mouse nepmucin.

Entrez Gene IDs

146894 (Human); 52685 (Mouse); 684984 (Rat)

Alternate Names

CD300 antigen like family member G; CD300 antigen-like family member G; CD300 molecule like family member g; CD300 molecule-like family member g; CD300g antigen; CD300g; CD300LG; CLM9; CLM-9; CLM9Trem4; CMRF35-like molecule 9; Nepmucin; TREM4; TREM-4; Triggering receptor expressed on myeloid cells 4