Human CD36/SR-B3 APC-conjugated Antibody

Catalog #: FAB19551A
1 Citation Datasheet / COA / SDS

Discontinued Product

FAB19551A has been discontinued.
View all CD36/SR-B3 products.
Detection of CD36/SR‑B3 in HepG2 Human Cell Line by Flow Cytometry.
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Citations (1)
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Human CD36/SR-B3 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD36/SR‑B3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse CD36 is observed.
Source
Monoclonal Rat IgG2B Clone # 255606
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD36/SR‑B3
Gly30-Asn439
Accession # P16671
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD36/SR-B3 antibody in HepG2 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of CD36/SR‑B3 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Rat Anti-Human CD36/SR-B3 APC-conjugated Monoclonal Antibody (Catalog # FAB19551A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD36/SR-B3

CD36, alternatively known as platelet membrane glycoprotein IV (GPIV), GPIIIb, thrombospondin receptor, collagen receptor, fatty acid translocase (FAT), and scavenger receptor class B, member 3 (SR-B3), is an integral membrane glycoprotein that has multiple physiological functions (1). It is broadly expressed on a variety of cell types including microvascular endothelium, adipocytes, skeletal muscle, epithelial cells of the retina, breast, and intestine, smooth muscle cells, erythroid precursors, platelets, megakaryocytes, dendritic cells, monocytes/macrophages, and microglia (1, 2). As a member of the scavenger receptor family, CD36 is a multiligand pattern recognition receptor that interacts with a large number of structurally dissimilar ligands, including long chain fatty acid (LCFA), advanced glycation end products (AGE), thrombospondin-1, oxidized low-density lipoproteins (oxLDLs), high density lipoprotein (HDL), phosphatidylserine, apoptotic cells, beta ‑amyloid fibrils (fA beta ), collagens I and IV, and Plasmodium falciparum-infected erythrocytes (3). CD36 is required for the anti-angiogenic effects of thrombospondin-1 in the corneal neovascularization assay (4). It plays a role in lipid metabolism and has been identified as a fatty acid translocase necessary for the binding and transport of LCFA in cells and tissues (5). CD36 has been implicated in the clearance of apoptotic cells and cell debris and has also been shown to mediate the internalization and degradation of a variety of its ligands such as oxLDL, AGE and fA beta (3). Upon ligand binding, CD36 transduces signals that mediate a wide range of pro-inflammatory cellular responses (2). CD36 plays a significant role in the initiation and pathogenesis of chronic inflammatory diseases such as Alzheimer’s disease and atherosclerosis (2, 3). The human CD36 gene encodes a single-chain 472 amino acid protein containing both an N- and a C-terminal cytoplasmic tail and an extracellular loop.

References
  1. Febbraio, M. et al. (2001) J. Clin. Invest. 108:785. 
  2. Khoury, J. et al. (2003) J. Exp. Med. 197:1657. 
  3. Husemann, J. et al. (2002) Glia 40:195.
  4. Armstrong, L and P. Bornstein (2003) Matrix. Biol. 22:63.
  5. Febbraio M. et al. (1999) J. Biol. Chem. 274:19055.
Long Name
Scavenger Receptor Class B, Member 3
Entrez Gene IDs
948 (Human); 12491 (Mouse)
Alternate Names
BDPLT10; CD_antigen: CD36; CD36 antigen (collagen type I receptor, thrombospondin receptor); CD36 antigen; CD36 molecule (thrombospondin receptor); CD36; CHDS7; cluster determinant 36; Collagen R; FAT; Fatty acid translocase; Glycoprotein IIIb; GP3B; GP4; GPIIIb; GPIV; PAS IV; PAS-4 protein; PAS-4; PASIV; Platelet collagen receptor; platelet glycoprotein 4; SCARB3; scavenger receptor class B, member 3; SRB3; SR-B3; Thrombospondin R; Thrombospondin receptor

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Citation for Human CD36/SR-B3 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. In vitro palmitate treatment of myotubes from postmenopausal women leads to ceramide accumulation, inflammation and affected insulin signaling.
    Authors: Abildgaard J, Henstridge D, Pedersen A, Langley K, Scheele C, Pedersen B, Lindegaard B
    PLoS ONE, 2014-07-07;9(7):e101555.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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