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Human CD81 Antibody

Catalog #: MAB4615
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4615
MAB4615-SP
Detection of CD81 in Human Lymphocytes by Flow Cytometry.
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Product Details
Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD81 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD81. Stains human CD81 transfectants but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2B Clone # 454720
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HEK293 human embryonic kidney cell line transfected with human CD81
Accession # P60033
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD81 antibody in Human Lymphocytes antibody by Flow Cytometry. View Larger

Detection of CD81 in Human Lymphocytes by Flow Cytometry. Human whole blood lymphocytes were stained with Mouse Anti-Human CD81 Monoclonal Antibody (Catalog # MAB4615, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD81

CD81, also known as TAPA-1 and TM4SF4, is a widely expressed protein in the tetraspanin family. CD81 is a multifunctional protein that interacts with a variety of other molecules, including tetraspanins, and is important for organization of the plasma membrane into microdomains. CD81 facilitates B cell and T cell activation and is an integrin-binding adhesion molecule. CD81 expression on lymphocytes is altered during infection by hepatitis C virus or HIV-1 and contributes to the pathogenicity of those viruses. Human CD81 shares 92%‑93% aa sequence identity with mouse and rat CD81.

Entrez Gene IDs
975 (Human); 12520 (Mouse); 25621 (Rat)
Alternate Names
CD81 antigen (target of antiproliferative 1); CD81 antigen; CD81 molecule; CD81; CVID6; TAPA1; TAPA-1; Target of the antiproliferative 1; Tetraspanin-28; TSPAN28; Tspan-28; tspan-28,26 kDa cell surface protein TAPA-1; TSPAN28S5.7

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Citations for Human CD81 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Selective immunocapture reveals neoplastic human mast cells secrete distinct microvesicle- and exosome-like populations of KIT-containing extracellular vesicles
    Authors: Pfeiffer A, Petersen JD, Falduto GH et al.
    Journal of extracellular vesicles
  2. Presentation of antigen on extracellular vesicles using transmembrane domains from viral glycoproteins for enhanced immunogenicity
    Authors: Kai Hu, Paul F. McKay, Karnyart Samnuan, Adrian Najer, Anna K. Blakney, Junyi Che et al.
    Journal of Extracellular Vesicles
  3. Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
    Authors: Q Zhang, DK Jeppesen, JN Higginboth, R Graves-Dea, VQ Trinh, MA Ramirez, Y Sohn, AC Neininger, N Taneja, ET McKinley, H Niitsu, Z Cao, R Evans, SE Glass, KC Ray, WH Fissell, S Hill, KL Rose, WJ Huh, MK Washington, GD Ayers, DT Burnette, S Sharma, LH Rome, JL Franklin, YA Lee, Q Liu, RJ Coffey
    Nature Cell Biology, 2021-12-09;23(12):1240-1254.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Characterization of ALTO-encoding circular RNAs expressed by Merkel cell polyomavirus and trichodysplasia spinulosa polyomavirus
    Authors: R Yang, EE Lee, J Kim, JH Choi, E Kolitz, Y Chen, C Crewe, NJH Salisbury, PE Scherer, C Cockerell, TR Smith, L Rosen, L Verlinden, DA Galloway, CB Buck, MC Feltkamp, CS Sullivan, RC Wang
    PloS Pathogens, 2021-05-17;17(5):e1009582.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Transfer of Functional Cargo in Exomeres
    Authors: Q Zhang, JN Higginboth, DK Jeppesen, YP Yang, W Li, ET McKinley, R Graves-Dea, J Ping, CM Britain, KA Dorsett, CL Hartman, DA Ford, RM Allen, KC Vickers, Q Liu, JL Franklin, SL Bellis, RJ Coffey
    Cell Rep, 2019-04-04;0(0):.
    Species: Human
    Sample Types: Exosomes
    Applications: Western Blot
  6. Thy-1 dependent uptake of mesenchymal stem cell-derived extracellular vesicles blocks myofibroblastic differentiation
    Authors: TP Shentu, TS Huang, M Cernelc-Ko, J Chan, SS Wong, CR Espinoza, C Tan, I Gramaglia, H van der He, S Chien, JS Hagood
    Sci Rep, 2017-12-22;7(1):18052.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Cell-based therapy using miR-302-367 expressing cells represses glioblastoma growth
    Authors: M Fareh, F Almairac, L Turchi, F Burel-Vand, P Paquis, D Fontaine, S Lacas-Gerv, MP Junier, H Chneiweiss, T Virolle
    Cell Death Dis, 2017-03-30;8(3):e2713.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Hepatocyte Nuclear Factor 4 alpha and Downstream Secreted Phospholipase A 2 GXIIB Regulate Production of Infectious Hepatitis C Virus
    Authors: Xinlei Li, Hanfang Jiang, Linbing Qu, Wenxia Yao, Hua Cai, Ling Chen et al.
    Journal of Virology

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