Human CD81 APC-conjugated Antibody

Name: Human CD81 APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB4615A

Catalog #: FAB4615A Datasheet / COA / SDS

Discontinued Product

FAB4615A has been discontinued.

View all CD81 products.

Detection of CD81 in Human Lymphocytes by Flow Cytometry.

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All CD81 Antibodies

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Human CD81 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD81. Stains human CD81 transfectants but not irrelevant transfectants.

Source

Monoclonal Mouse IgG_2B Clone # 454720

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

HEK293 human embryonic kidney cell line transfected with human CD81
Accession # P60033

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD81 in Human Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD81 APC-conjugated Monoclonal Antibody (Catalog # FAB4615A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD81

CD81, also known as TAPA-1 and TM4SF4, is a widely expressed protein in the tetraspanin family. CD81 is a multifunctional protein that interacts with a variety of other molecules, including tetraspanins, and is important for organization of the plasma membrane into microdomains. CD81 facilitates B cell and T cell activation and is an integrin-binding adhesion molecule. CD81 expression on lymphocytes is altered during infection by hepatitis C virus or HIV-1 and contributes to the pathogenicity of those viruses. Human CD81 shares 92%‑93% aa sequence identity with mouse and rat CD81.

Entrez Gene IDs

975 (Human); 12520 (Mouse); 25621 (Rat)

Alternate Names

CD81 antigen (target of antiproliferative 1); CD81 antigen; CD81 molecule; CD81; CVID6; TAPA1; TAPA-1; Target of the antiproliferative 1; Tetraspanin-28; TSPAN28; Tspan-28; tspan-28,26 kDa cell surface protein TAPA-1; TSPAN28S5.7