Human CDNF Biotinylated Antibody

Catalog #: BAF5097 Datasheet / COA / SDS

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BAF5097

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Human CDNF Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CDNF in Western blots. In Western blots, approximately 10% cross‑reactivity with recombinant mouse CDNF is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CDNF
Gln25-Leu187
Accession # Q49AH0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human CDNF (Catalog # 5097-CD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CDNF

CDNF (conserved dopamine neurotrophic factor), also called Armetl1 (arginine-rich, mutated in early stage tumors-like 1) in mouse, is a 17-19 kDa secreted protein that shares 62% amino acid (aa) identity with human MANF (mesencephalic-astrocyte-derived neurotrophic factor), also called Armet in mouse (1). The Armet designation is not preferred, because the proteins when translated are not actually arginine-rich (1). However, both CDNF and MANF have a high proportion of charged residues, a pattern of eight cysteines shown to form intramoleculular disulfides, and a C-terminal endoplasmic reticulum retention signal (shown to function in MANF) (1-3). The human CDNF cDNA encodes a 187 aa protein with a 24 aa signal sequence and a 163 mature sequence (1). Mature human CDNF shares 80%, 84%, 90%, and 92% aa identity with mouse, rat, equine, and bovine CDNF, respectively. Although CDNF mRNA and protein are expressed in pre- and post-natal mouse brain, they are most abundant in adult heart, skeletal muscle and testis. Transcripts within the postnatal mouse brain are concentrated in the hippocampus, thalamus, corpus callosum and optic nerve (1). Like MANF and GDNF, CDNF promotes survival of dopaminergic neurons in vitro (1, 4). In a rat Parkinson’s disease model, CDNF also promotes rescue and restoration of dopaminergic neurons in vivo (1).

References

Lindholm, P. et al. (2007) Nature 448:73.
Mizobuchi, N. et al. (2007) Cell Struct. Funct. 32:41.
Raykhel, I. et al. (2007) J. Cell Biol. 179:1193.
Petrova, P. et al. (2003) J. Mol. Neurosci. 20:173.

Long Name

Conserved Dopamine Neurotrophic Factor

Entrez Gene IDs

441549 (Human); 227526 (Mouse); 361276 (Rat)

Alternate Names

arginine-rich, mutated in early stage tumors-like 1; ARMETL1; ARMET-like protein 1; CDNF; cerebral dopamine neurotrophic factor; Conserved dopamine neurotrophic factor