Human CDP/CUTL1 Antibody

Name: Human CDP/CUTL1 Antibody
Brand: R&D Systems
SKU: AF4756
Rating: 5 (1 reviews)

Catalog #: AF4756 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4756
AF4756-SP

Detection of Human CDP/CUTL1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CDP/CUTL1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CDP/CUTL1 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human CDP/CUTL1
Ile64-Ser268
Accession # P39880

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human CDP/CUTL1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CDP/CUTL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4756) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for CDP/CUTL1 at approximately 190 kDa and 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CDP/CUTL1

CDP (CCAAT Displacement Protein; also CUX-1 and CUTL-1) is a 180-190 kDa member of the CUT homeobox family of proteins. It is found in multiple cell types, and appears to act as a negative regulator of transcription for genes as diverse as c-myc, CD8 and TGF-beta RII. Repression is assumed to occur either by direct DNA interaction, which interferes with transcriptional activator binding to gene promoters, or through the recruitment of active HDACs to target gene promoter sequences. Human CDP is 1505 amino acids (aa) in length. It contains three consecutive DNA-binding CUT domains (aa 542-629; 934-1007; 1125-1202) plus one C-terminal homeobox domain (aa 1244-1303). CDP undergoes phosphorylation, resulting in its inactivation, and reportedly also homodimerizes. There are multiple splice variants (termed CASP), ranging from 75-85 kDa in size. Most are characterized by the presence of a unique 260 aa substitution for aa 410-1505 that contains a Golgi transmembrane domain. CDP also undergoes extensive proteolytic processing, creating functional DNA binding C-terminal fragments that range from 75-110 kDa in size. Over aa 64-268, human CDP shares 97% aa identity with mouse CDP.

Long Name

CCAAT Displacement protein/CUT-like Homeobox Protein 1

Entrez Gene IDs

1523 (Human); 13047 (Mouse); 116639 (Rat)

Alternate Names

CASP; CCAAT displacement protein; CDP; CDP/Cux; CDPCCAAT displacement protein; COY1; cut (Drosophila)-like 1 (CCAAT displacement protein); CUT; CUTL1; cut-like 1, CCAAT displacement protein (Drosophila); cut-like homeobox 1; CUX; CUX1; FLJ31745; golgi integral membrane protein 6; GOLIM6; homeobox protein cut-like 1; Homeobox protein cux-1; Nbla10317; p100; p110; p200; p75; protein CASP; putative protein product of Nbla10317