Human CEACAM-16 Alexa Fluor® 647-conjugated Antibody

Catalog #: FAB9885R Datasheet / COA / SDS

Recombinant Monoclonal Antibody

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FAB9885R-100UG

All CEACAM-16 Antibodies

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Human CEACAM-16 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CEACAM-16 in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2747B

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Chinese Hamster Ovary cell line CHO-derived human CEACAM-16
Met1-Gly425
Accession # Q2WEN9

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

Titration recommended for optimal concentrationwith starting range of 0.1-1 µg/1 million cells. Sample used for thisexperiment was HEK293 Human Cell Line Transfected with Human CEACAM-16.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CEACAM-16

Carcinoembryonic Antigen-related Cell Adhesion Molecule 16 (CEACAM-16), or CEAL2, is part of the CEA protein family consisting of CEACAMs and the pregnancy‑specific glycoproteins (PSGs). Both CEACAM and PSG molecules have been identified in humans and belong to the much larger glycosylphosphatidylinositol (GPI)-linked immunoglobulin (Ig) superfamily (1, 2). Unique to the CEA family, CEACAM-16 is a secreted molecule lacking a recognizable transmembrane domain or GPI anchor (3). Mature human CEACAM-16 is 405 amino acids (aa), containing 2 IgC2-like domains and 2 IgV-like domains. CEACAM-16 is one of only five conserved CEACAMs among mice, rats, and humans (2). Mature human CEACAM-16 shares 90% and 89% aa identity with mouse and rat CEACAM‑16, respectively. Originally discovered as a biomarker for colorectal cancer (4), CEACAMs have now been associated with numerous intracellular signaling processes including cell adhesion, cell growth, recognition and differentiation, angiogenesis, and apoptosis (5-7). CEACAM-16 is specifically expressed in the inner ear and has been shown to play a critical role in hearing. CEACAM-16 has been identified as a binding partner for alpha tectorin and specific mutations in CEACAM-16 have been linked to autosomal dominant nonsyndromic deafness (ADNSHL) (3, 8).

References

Beauchemin, N. et al. (1999) Exp. Cell Res. 252:243.
Zebhauser R. et al. (2005) Genomics 86:566.
Zheng, J. et al. (2011) PNAS. 108(10):4218.
Gold P. and Freedman S.O. (1965) J Exp Med 122:467.
Obrink, B. (1997) Curr Opin Cell Biol 9:616.
Horst, A.K. and Wagener, C. (2004) Handb Exp Pharmacol 283.
Kuespert K et al. (2006) Curr Opin Cell Biol. 18(5):565.
Wang, H. et al. (2015) J Hum Genet. 60(3):119.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 16

Entrez Gene IDs

388551 (Human); 330483 (Mouse); 292700 (Rat)

Alternate Names

carcinoembryonic antigen-related cell adhesion molecule 16; CEACAM-16; CEAL2; DFNA4B

Product Datasheets

Product Datasheet

COA

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Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.