Human CEBP alpha Antibody

Name: Human CEBP alpha Antibody
Brand: R&D Systems
SKU: MAB7094
Rating: 5 (2 reviews)

Catalog #: MAB7094 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7094
MAB7094-SP

Detection of Human CEBP alpha by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CEBP alpha Antibody Summary

Species Reactivity

Human

Specificity

Detects human CEBP alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CEBP beta, gamma, delta, epsilon, or zeta is observed.

Source

Monoclonal Mouse IgG_2B Clone # 692716

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human CEBP alpha
Met1-Ala124
Accession # P49715

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human CEBP alpha by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human CEBP a Monoclonal Antibody (Catalog # MAB7094) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CEBP a at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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CEBP alpha in Human Mesenchymal Stem Cells. CEBP a was detected in immersion fixed human mesenchymal stem cells using Mouse Anti-Human CEBP a Monoclonal Antibody (Catalog # MAB7094) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CEBP alpha

CCAAT-enhancer binding protein alpha (CEBP alpha ) is a widely expressed 42 kDa basic-leucine zipper transcription factor that forms functional homodimers and heterodimers with CEBP beta and CEBP gamma. CEBP alpha inhibits mitotic progression through multiple mechanisms and also promotes terminal differentiation of granulocytes and adipocytes. Its activity is regulated by SUMOylation and by phosphorylation at multiple serine residues. CEBP alpha contains three TE transactivation domains (aa 1‑69, aa 70-96, and aa 126-200) and one basic DNA binding motif and leucine zipper (aa 286 - 345). A 30 kDa isoform is generated by the use of an internal translation initiation site. The p30 isoform functions as a dominant negative factor by heterodimerizing with and inhibiting full length CEBP alpha activity. Within aa 1‑124, human CEBP alpha shares 93% amino acid sequence identity with mouse and rat CEBP alpha, respectively.

Long Name

CCAAT/enhancer-Binding Protein alpha

Entrez Gene IDs

1050 (Human); 12606 (Mouse); 24252 (Rat)

Alternate Names

C/EBP alpha; C/EBP-alpha; CCAAT/enhancer binding protein (C/EBP), alpha; CCAAT/enhancer-binding protein alpha; CEBP alpha; CEBP; CEBPA