Human Cerebellin-2 Antibody

Catalog #: MAB7044 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7044
MAB7044-SP

Detection of Human and Mouse Cerebellin-2 by Western Blot.

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All Cerebellin-2 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Cerebellin-2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Cerebellin-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Cerebellin-1, -3, or -4 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 704330

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Cerebellin-2
Arg50-Leu224
Accession # Q8IUK8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse Cerebellin-2 by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue, human brain (cortex) tissue, human brain (hippocampus) tissue, human brain (hypothalamus) tissue, and mouse brain (cerebellum) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Cerebellin-2 Monoclonal Antibody (Catalog # MAB7044) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cerebellin-2 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cerebellin-2

Cerebellin-2 (CBLN2) is an approximately 25 kDa glycoprotein in the Cerebellin family of C1q- and TNF-related proteins. It contains a potential membrane-associating segment but may also be secreted. It contains one C1q-like domain in its extracellular region. Cerebellin-2 expression is developmentally regulated in the cerebellum, neocortex, sensory neurons of the dorsal root ganglion and spinal cord, and adrenal cortex. It associates with Cerebellin-1 which is involved in the formation and maintenance of synapses in the cerebellum. Within the ectodomain (aa 50-224), human Cerebellin-2 shares 99% aa sequence identity with mouse and rat Cerebellin-2.

Entrez Gene IDs

147381 (Human); 12405 (Mouse); 291388 (Rat)

Alternate Names

CBLN2; cerebellin 2 precursor; Cerebellin2; Cerebellin-2