Human Chemerin Antibody

Catalog #: MAB23241
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB23241-SP
MAB23241-500
MAB23241-100

All Chemerin Antibodies

Product Details

Citations (3)

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Human Chemerin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Chemerin in ELISAs. In ELISAs, this antibody does not cross-react with recombinant mouse Chemerin.

Source

Monoclonal Mouse IgG_2B Clone # 365314

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Chemerin
Glu21-Ser157
Accession # Q99969

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Human Chemerin Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human Chemerin Biotinylated Antibody (Catalog # BAF2324)

Standard: Recombinant Human Chemerin Protein (Catalog # 2324-CM)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chemerin

Human Chemerin, also known as Tazarotene-induced Gene 2, (TIG2) is a new, but distant member of the Cystatin superfamily (1-3). Members of this superfamily contain at least two intrachain disulfide bonds and an alpha -helical structure over a distance of about 100 amino acids (2, 3). Chemerin is synthesized as a 163 aa precursor that contains a hydrophobic 20 aa N-terminal sequence, an intervening 137 aa Cystatin-fold containing domain, and a six aa C-terminal prosegment (1, 4). Within the cystatin-fold domain there are three intrachain disulfide bonds that contribute to the fold, and three potential sites for phosphorylation and one for myristoylation (5). The precursor molecule undergoes proteolytic processing at both termini by unknown proteases. The N-terminal residue 20 aa hydrophobic segment is described as being either a signal sequence or a transmembrane (TM) segment for a type II TM protein (1, 6). In either case, it gives rise to a soluble proform that undergoes further processing at the C-terminus. In human, the C-terminal six residues are cleaved, giving rise to a monomeric, 16 kDa heparin-binding bioactive molecule (aa 21-157) (7). A shorter 134 aa form has been described (5). Bioactivity seems to be concentrated in the nine residues preceding the prosegment (aa 149‑157). Retention of the prosegment blocks activity (4). The 137 aa mature segment is known to bind to the G-protein coupled receptor termed ChemR23 (5, 7). Binding results in macrophage and immature dendritic cell chemotaxis (7). The distribution of this receptor is limited to immune APCs, and it is assumed that Chemerin is an inflammatory molecule. It is unclear which cells are actually producing Chemerin, but keratinocytes, endothelial cells and osteoclasts are potential candidates (1, 7). Mature human Chemerin shares 67% aa sequence identity with mouse Chemerin (7). There is apparently cross-species activity for the protein (8).

References

Nagpal, S. et al., (1997) J. Invest. Dermatol. 109:91.
Storici, P. et al., (1996) Eur. J. Biochem. 238:769.
Zanetti, M., (2004) J. Leukoc. Biol. 75:39.
Wittamer, V. et al., (2004) J. Biol. Chem. 279:9956.
Meder, W. et al., (2003) FEBS Lett. 555:495.
Yokoyama-Kobayashi, M. et al., (1999) Gene 228:161.
Wittamer, V. et al., (2003) J. Exp. Med. 198:977.
Busmann, A. et al., (2004) J. Chromatog. B 811:217.

Long Name

Retinoic Acid Receptor Responder Protein 2

Entrez Gene IDs

5919 (Human); 71660 (Mouse)

Alternate Names

Chemerin; RARRES2; RAR-responsive protein TIG2; retinoic acid receptor responder (tazarotene induced) 2; retinoic acid receptor responder protein 2; Tazarotene-induced gene 2 protein; TIG-2; TIG2HP10433

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Citations for Human Chemerin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Chemerin 156F, generated by chymase cleavage of prochemerin, is elevated in joint fluids of arthritis patients
Authors: L Zhao, Y Yamaguchi, X Ge, WH Robinson, J Morser, LLK Leung
Arthritis Res. Ther., 2018-07-04;20(1):132.
Species: Human
Sample Types: Protein
Applications: ELISA Development (Capture)
Antimicrobial and Attractant Roles for Chemerin in the Oral Cavity during Inflammatory Gum Disease
Authors: U Godlewska, P Brzoza, A Sroka, P Majewski, H Jentsch, M Eckert, S Eick, J Potempa, BA Zabel, J Cichy
Front Immunol, 2017-03-29;8(0):353.
Species: Human
Sample Types: Serum
The expression and regulation of chemerin in the epidermis.
Authors: Banas M, Zegar A, Kwitniewski M, Zabieglo K, Marczynska J, Kapinska-Mrowiecka M, LaJevic M, Zabel B, Cichy J
PLoS ONE, 2015-02-06;10(2):e0117830.
Species: Human
Sample Types: Tissue Homogenates
Applications: ELISA Development (Capture)