Human Chitinase 3-like 2 Antibody

Catalog #: MAB5116 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5116
MAB5116-SP

Detection of Human Chitinase 3-like 2 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Chitinase 3-like 2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Chitinase 3-like 2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Chitinase 3‑like 1 or rhChitotriosidase is observed.

Source

Monoclonal Mouse IgG_2B Clone # 632010

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Chitinase 3-like 2
Tyr27-Leu390
Accession # Q15782

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Chitinase 3-like 2 by Western Blot. Western blot shows lysates of human lung tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human Chitinase 3-like 2 Monoclonal Antibody (Catalog # MAB5116) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Chitinase 3-like 2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chitinase 3-like 2

Chitinase 3-like 2 (CHI3L2; also chrondrocyte protein 39 and YKL-39) is a 39 kDa glycoprotein member of the glycosyl hydrolase 18 family. It is secreted by synovial fibroblasts and chondrocytes, particularly in osteoarthritis, and may contribute to extracellular matrix stability. CHI3L2 has no chitotriosidase activity, but is likely to bind some type of glycan. The human CHI3L2 precursor is 390 amino acids (aa) in length. It contains a 26 aa signal sequence and a 364 aa mature region. There are two potential alternate start sites at Met24 and Met80; a third potential isoform shows a deletion of aa 15‑24. Over aa 27‑390, human CHI3L2 shares 89% aa identity with bovine CHI3L2. No rodent CHI3L2 has been reported.

Entrez Gene IDs

1117 (Human)

Alternate Names

CHI3L2; Chitinase 3 like 2; Chitinase 3-like 2; chitinase-3-like protein 2; Chondrocyte protein 39; YKL39; YKL-39YKL39