Human Chitobiase/CTBS Antibody

Catalog #: AF4829 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4829
AF4829-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Chitobiase/CTBS Antibody Summary

Species Reactivity

Human

Specificity

Detects human Chitobiase/CTBS in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Chitobiase/CTBS
Thr39-Arg385
Accession # Q01459

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Chitobiase/CTBS

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chitobiase/CTBS

CTBS (Di-N-acetylchitobiase; also chitobiase) is a 40 kDa lysosomal glycoprotein that belongs to glycosyl hydrolase family # 18. It is a reducing-end glycoside hydrolase that serves to degrade asparagines-linked carbohydrates. Mature human CTBS is 347 amino acids (aa) in length and contains one catalytic domain (aa 115‑357). There are multiple potential isoforms of CTBS. One shows a three aa deletion of aa 30‑32, accompanied by a two two aa substitution for the C-terminal 279 amino acids. There are two potential alternate start sites, one at Met92 and a second at Met207. Mature human CTBS (aa 39‑385) is 85% aa identical to mouse CTBS.

Entrez Gene IDs

1486 (Human); 74245 (Mouse); 81652 (Rat)

Alternate Names

Chitobiase; chitobiase, di-N-acetyl-; CTBdi-N-acetylchitobiase; CTBS; EC 3.2.1.-